Silencing of quinolinic acid phosphoribosyl transferase (QPT) gene for enhanced production of scopolamine in hairy root culture of Duboisia leichhardtii.

Autor: Singh P; Plant Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), P.O. CIMAP, Picnic Spot Road, Lucknow, U.P., 226015, India.; Academy of Scientific and Innovative Research (AcSIR), CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, 226015, India., Prasad R; Plant Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), P.O. CIMAP, Picnic Spot Road, Lucknow, U.P., 226015, India., Tewari R; Chemical Science Division, Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Picnic Spot Road, Lucknow, U.P., 226015, India., Jaidi M; Plant Molecular Virology Laboratory, CPMB Division, National Botanical Research Institute, Lucknow, U.P., 226001, India., Kumar S; Plant Molecular Virology Laboratory, CPMB Division, National Botanical Research Institute, Lucknow, U.P., 226001, India., Rout PK; Chemical Science Division, Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Picnic Spot Road, Lucknow, U.P., 226015, India., Rahman LU; Plant Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), P.O. CIMAP, Picnic Spot Road, Lucknow, U.P., 226015, India. l.rahman@cimap.res.in.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2018 Sep 17; Vol. 8 (1), pp. 13939. Date of Electronic Publication: 2018 Sep 17.
DOI: 10.1038/s41598-018-32396-0
Abstrakt: Scopolamine is a pharmaceutically important tropane alkaloid which is used therapeutically in the form of an anesthetic and antispasmodic drug. The present study demonstrates enhanced scopolamine production from transgenic hairy root clones of Duboisia leichhardtii wherein the expression of quinolinate phosphoribosyl transferase (QPT) gene was silenced using the QPT-RNAi construct under the control of CaMV 35 S promoter. The RNAi hairy roots clones viz. P4, P7, P8, and P12 showed the enhanced synthesis of scopolamine with significant inhibition of nicotine biosynthesis. Optimization of culture duration in combination with methyl jasmonate elicitor in different concentrations (50 µM-200 µM) was carried out. Maximum synthesis of scopolamine had obtained from HR clones P7 (8.84 ± 0.117 mg/gm) on the 30 th day of cultivation. Conspicuously, elicitation with wound-associated hormone methyl jasmonate enhanced the yield of scopolamine 2.2 fold (19.344 ± 0.275 mg/gm) compared to the culture lacking the elicitor. The transgenic hairy roots cultures established with RNAi mediated silencing of quinolinate phosphoribosyl transferase gene provides an alternative approach to increase the yield of scopolamine in fulfilling the demand of this secondary metabolite.
Databáze: MEDLINE
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