A rapid LC-MS method for qualitative and quantitative profiling of plant apocarotenoids.
Autor: | Mi J; King Abdullah University of Science and Technology (KAUST), Biological and Environmental Sciences and Engineering Division, The Bioactives Lab, Thuwal 23955-6900, Saudi Arabia., Jia KP; King Abdullah University of Science and Technology (KAUST), Biological and Environmental Sciences and Engineering Division, The Bioactives Lab, Thuwal 23955-6900, Saudi Arabia., Wang JY; King Abdullah University of Science and Technology (KAUST), Biological and Environmental Sciences and Engineering Division, The Bioactives Lab, Thuwal 23955-6900, Saudi Arabia., Al-Babili S; King Abdullah University of Science and Technology (KAUST), Biological and Environmental Sciences and Engineering Division, The Bioactives Lab, Thuwal 23955-6900, Saudi Arabia. Electronic address: salim.babili@kaust.edu.sa. |
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Jazyk: | angličtina |
Zdroj: | Analytica chimica acta [Anal Chim Acta] 2018 Dec 04; Vol. 1035, pp. 87-95. Date of Electronic Publication: 2018 Jul 03. |
DOI: | 10.1016/j.aca.2018.07.002 |
Abstrakt: | Carotenoid cleavage products (apocarotenoids; APOs) exert important biological functions in light perception and as vitamin A source, signaling molecules, hormone precursors, pigments and volatiles. However, an analytical method that allows simultaneous profiling of these diverse compounds is still missing. We developed an efficient method to analyze APOs present in plant tissues, which is based on ultra-high performance liquid chromatographic separation and high-resolution hybrid quadrupole-Orbitrap (Q-Orbitrap) mass spectrometry (MS). Our approach allowed unambiguous identification and quantification of volatile and non-volatile APOs in a single run. Modified sample preparation and optimized ultra-high performance liquid chromatography (UHPLC)-MS parameters permitted the measurement of APOs in Oryza sativa seedlings and Spinacia oleracea leaves, unraveling 20 endogenous APOs with chain lengths ranging from C (Copyright © 2018 Elsevier B.V. All rights reserved.) |
Databáze: | MEDLINE |
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