Six domesticated PiggyBac transposases together carry out programmed DNA elimination in Paramecium .

Autor:	Bischerour J; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France., Bhullar S; Institute of Cell Biology, University of Bern, Bern, Switzerland.; Institut de Biologie de l'Ecole Normale Supérieure, Paris, France., Denby Wilkes C; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France., Régnier V; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France.; Univ Paris Diderot, Paris, France., Mathy N; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France., Dubois E; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France., Singh A; Institute of Cell Biology, University of Bern, Bern, Switzerland., Swart E; Institute of Cell Biology, University of Bern, Bern, Switzerland., Arnaiz O; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France., Sperling L; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France., Nowacki M; Institute of Cell Biology, University of Bern, Bern, Switzerland., Bétermier M; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2018 Sep 18; Vol. 7. Date of Electronic Publication: 2018 Sep 18.
DOI:	10.7554/eLife.37927
Abstrakt:	The domestication of transposable elements has repeatedly occurred during evolution and domesticated transposases have often been implicated in programmed genome rearrangements, as remarkably illustrated in ciliates. In Paramecium , PiggyMac (Pgm), a domesticated PiggyBac transposase, carries out developmentally programmed DNA elimination, including the precise excision of tens of thousands of gene-interrupting germline Internal Eliminated Sequences (IESs). Here, we report the discovery of five groups of distant Pgm-like proteins (PgmLs), all able to interact with Pgm and essential for its nuclear localization and IES excision genome-wide. Unlike Pgm, PgmLs lack a conserved catalytic site, suggesting that they rather have an architectural function within a multi-component excision complex embedding Pgm. PgmL depletion can increase erroneous targeting of residual Pgm-mediated DNA cleavage, indicating that PgmLs contribute to accurately position the complex on IES ends. DNA rearrangements in Paramecium constitute a rare example of a biological process jointly managed by six distinct domesticated transposases. Competing Interests: JB, SB, CD, VR, NM, ED, AS, ES, OA, LS, MN, MB No competing interests declared (© 2018, Bischerour et al.)
Databáze:	MEDLINE
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