Combining Butyrated ManNAc with Glycoengineered CHO Cells Improves EPO Glycan Quality and Production.
Autor: | Wang Q; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA., Chung CY; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA., Yang W; Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA., Yang G; Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA., Chough S; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA., Chen Y; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA., Yin B; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA., Bhattacharya R; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21231, USA., Hu Y; Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA., Saeui CT; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21231, USA., Yarema KJ; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21231, USA., Betenbaugh MJ; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA., Zhang H; Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA. |
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Jazyk: | angličtina |
Zdroj: | Biotechnology journal [Biotechnol J] 2019 Apr; Vol. 14 (4), pp. e1800186. Date of Electronic Publication: 2018 Oct 08. |
DOI: | 10.1002/biot.201800186 |
Abstrakt: | Sodium butyrate (NaBu) is not only well-known for enhancing protein production, but also degrades glycan quality. In this study, butyrate supplied by the precursor molecule 1,3,4-O-Bu (© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.) |
Databáze: | MEDLINE |
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