Oncogenic microRNA signature for early diagnosis of cervical intraepithelial neoplasia and cancer.

Autor: Liu SS; Department of Obstetrics & Gynaecology, LKS Faculty of Medicine, The University of Hong Kong, SAR, China., Chan KKL; Department of Obstetrics & Gynaecology, LKS Faculty of Medicine, The University of Hong Kong, SAR, China., Chu DKH; Department of Obstetrics & Gynaecology, LKS Faculty of Medicine, The University of Hong Kong, SAR, China., Wei TN; Department of Obstetrics & Gynaecology, LKS Faculty of Medicine, The University of Hong Kong, SAR, China., Lau LSK; Department of Obstetrics & Gynaecology, LKS Faculty of Medicine, The University of Hong Kong, SAR, China., Ngu SF; Department of Obstetrics & Gynaecology, LKS Faculty of Medicine, The University of Hong Kong, SAR, China., Chu MMY; Department of Obstetrics & Gynaecology, LKS Faculty of Medicine, The University of Hong Kong, SAR, China., Tse KY; Department of Obstetrics & Gynaecology, LKS Faculty of Medicine, The University of Hong Kong, SAR, China., Ip PPC; Department of Pathology, LKS Faculty of Medicine, Queen Mary Hospital, The University of Hong Kong, SAR, China., Ng EKO; Pangenia Lifesciences Limited, Billion Centre, Hong Kong, SAR, China., Cheung ANY; Department of Pathology, LKS Faculty of Medicine, Queen Mary Hospital, The University of Hong Kong, SAR, China., Ngan HYS; Department of Obstetrics & Gynaecology, LKS Faculty of Medicine, The University of Hong Kong, SAR, China.
Jazyk: angličtina
Zdroj: Molecular oncology [Mol Oncol] 2018 Dec; Vol. 12 (12), pp. 2009-2022. Date of Electronic Publication: 2018 Sep 27.
DOI: 10.1002/1878-0261.12383
Abstrakt: Cervical cancer is one of the leading causes of cancer death in women globally, despite the widespread use of cytology/human papillomavirus (HPV) screening. In the present study, we aimed to identify the potential role of microRNA (miRNA) as a diagnostic biomarker in the detection of cervical pre-malignant lesions and cancer. In total, we recruited 582 patients with cervical diseases and 145 control individuals. The expression levels of six miRNAs (miR-20a, miR-92a, miR-141, miR-183*, miR-210 and miR-944) were found to be significantly up-regulated in cervical cancer and pre-malignant lesions compared to normal cervical samples, indicating that they are oncogenic miRNAs. Receiver operating characteristic curve analysis showed that these six miRNAs can be used to distinguish patients with cervical pre-malignant lesions or cancer from normal individuals and they also had a good predictive performance, particularly in cervical lesions. Combined use of these six miRNAs further enhanced the diagnostic accuracy over any single miRNA marker, with an area under the curve of 0.998, 0.996 and 0.959, a diagnostic sensitivity of 97.9%, 97.2% and 91.4%, and a specificity of 98.6%, 96.6% and 87.6% for low-grade lesions, high-grade lesions and cancer, respectively. This six oncogenic miRNA signature may be suitable for use as diagnostic marker for cervical pre-malignant lesions and cancer in the near future.
(© 2018 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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