The ZZ-type zinc finger of ZZZ3 modulates the ATAC complex-mediated histone acetylation and gene activation.

Autor: Mi W; Department of Epigenetics and Molecular Carcinogenesis, Center for Cancer Epigenetics, The University of Texas MD Anderson Cancer Center, Houston, Texas, 77030, USA.; Center for Epigenetics, Van Andel Research Institute, Grand Rapids, Michigan, 49503, USA., Zhang Y; Department of Pharmacology, University of Colorado School of Medicine, Aurora, Colorado, 80045, USA., Lyu J; Dan L. Duncan Cancer Center, Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, 77030, USA., Wang X; Department of Epigenetics and Molecular Carcinogenesis, Center for Cancer Epigenetics, The University of Texas MD Anderson Cancer Center, Houston, Texas, 77030, USA.; Center for Epigenetics, Van Andel Research Institute, Grand Rapids, Michigan, 49503, USA., Tong Q; Department of Pharmacology, University of Colorado School of Medicine, Aurora, Colorado, 80045, USA., Peng D; Department of Epigenetics and Molecular Carcinogenesis, Center for Cancer Epigenetics, The University of Texas MD Anderson Cancer Center, Houston, Texas, 77030, USA., Xue Y; Department of Epigenetics and Molecular Carcinogenesis, Center for Cancer Epigenetics, The University of Texas MD Anderson Cancer Center, Houston, Texas, 77030, USA., Tencer AH; Department of Pharmacology, University of Colorado School of Medicine, Aurora, Colorado, 80045, USA., Wen H; Department of Epigenetics and Molecular Carcinogenesis, Center for Cancer Epigenetics, The University of Texas MD Anderson Cancer Center, Houston, Texas, 77030, USA.; Center for Epigenetics, Van Andel Research Institute, Grand Rapids, Michigan, 49503, USA., Li W; Dan L. Duncan Cancer Center, Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, 77030, USA., Kutateladze TG; Department of Pharmacology, University of Colorado School of Medicine, Aurora, Colorado, 80045, USA. tatiana.kutateladze@ucdenver.edu., Shi X; Department of Epigenetics and Molecular Carcinogenesis, Center for Cancer Epigenetics, The University of Texas MD Anderson Cancer Center, Houston, Texas, 77030, USA. xiaobing.shi@vai.org.; Center for Epigenetics, Van Andel Research Institute, Grand Rapids, Michigan, 49503, USA. xiaobing.shi@vai.org.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2018 Sep 14; Vol. 9 (1), pp. 3759. Date of Electronic Publication: 2018 Sep 14.
DOI: 10.1038/s41467-018-06247-5
Abstrakt: Recognition of histones by epigenetic readers is a fundamental mechanism for the regulation of chromatin and transcription. Most reader modules target specific post-translational modifications on histones. Here, we report the identification of a reader of histone H3, the ZZ-type zinc finger (ZZ) domain of ZZZ3, a subunit of the Ada-two-A-containing (ATAC) histone acetyltransferase complex. The solution NMR structure of the ZZ in complex with the H3 peptide reveals a unique binding mechanism involving caging of the N-terminal Alanine 1 of histone H3 in an acidic cavity of the ZZ domain, indicating a specific recognition of H3 versus other histones. Depletion of ZZZ3 or disruption of the ZZ-H3 interaction dampens ATAC-dependent promoter histone H3K9 acetylation and target gene expression. Overall, our study identifies the ZZ domain of ZZZ3 as a histone H3 reader that is required for the ATAC complex-mediated maintenance of histone acetylation and gene activation.
Databáze: MEDLINE