| Autor: |
da Costa GCV; Laboratório de Apoio ao Desenvolvimento Tecnológico, Universidade Federal do Rio de Janeiro, Av. Horácio Macedo, Rio de Janeiro, RJ, 1281, Brazil., Peralta RHS; Departamento de Patologia, Faculdade de Medicina, Universidade Federal Fluminense, Rua Marques do Paraná, Niterói, RJ, 303, Brazil., Kalume DE; Fundação Oswaldo Cruz - Fiocruz, Instituto Oswaldo Cruz, Av. Brasil, Rio de Janeiro, RJ, Brazil., Alves ALGM; Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Av. Carlos Chagas Filho, Rio de Janeiro, RJ, 373, Brazil., Peralta JM; Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Av. Carlos Chagas Filho, Rio de Janeiro, RJ, 373, Brazil. peralta@micro.ufrj.br. |
| Abstrakt: |
The taeniasis/cysticercosis complex is a zoonosis caused by the presence of the parasite Taenia solium in humans. It is considered a neglected disease that causes serious public health and economic problems in developing countries. In humans, the most common locations for the larval form are the skeletal muscles, ocular system, and the central nervous system, which is the most clinically important. Several glycoproteins of T. solium and Taenia crassiceps cysticerci have been characterized and studied for their use in the immunodiagnosis of neurocysticercosis and/or the development of synthetic or recombinant vaccines against cysticercosis. The aim of this study was to perform a gel-free shotgun proteomic analysis to identify saline vesicular extract (SVE) proteins of T. solium and T. crassiceps cysticerci. After solubilization of the SVE with and without surfactant reagent and in-solution digestion, the proteins were analyzed by LC-MS/MS. Use of a surfactant resulted in a significantly higher number of proteins that were able to be identified by LC-MS/MS. Novel proteins were identified in T. solium and T. crassiceps SVE. The qualitative analysis revealed a total of 79 proteins in the Taenia species: 29 in T. solium alone, 11 in T. crassiceps alone, and 39 in both. These results are an important contribution to support future investigations and for establishing a Taenia proteomic profile to study candidate biomarkers involved in the diagnosis or pathogenesis of neurocysticercosis. |
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