[Sijunzi Decoction Promote the Repair of Intestinal Epithelial Cell Injury via Activating TLR-2/My D88 Signaling Pathway].

Autor:	Song HP, Li RY, Huang HY, Cai X, Yuan ZY, Liu PA, Zhou C
Jazyk:	čínština
Zdroj:	Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials [Zhong Yao Cai] 2016 Sep; Vol. 39 (9), pp. 2081-5.
Abstrakt:	Objective: To study the effect and mechanism of Sijunzi decoction( SJZD) containing serum on the repairing of gastrointestinal mucosal damage. Methods: SJZD containing serum was prepared by serum pharmacological method. Cell migration model was established by tips scratch method, Real-time-cell-analyzer( RTCA) was used to mensurate IEC-6 cell proliferation, the mRNA and protein expression of TLR-2 and My D88 mRNA were detected by qRT-PCR and Western blot analysis,respectively. Results: Medium dose( 10%) and high dose( 20%) of SJZD containing serum stimulated IEC-6 cell migration at 8 h after cell damage; medium dose( 10%)and high dose( 20%) of SJZD containing serum increased IEC-6 cell proliferation at 12 h after cell damage; low dose( 5%),medium dose( 10%) and high dose( 20%) of SJZD containing serum enhanced IEC-6 proliferation both at 24 h and 36 h after cell damage. Medium dose( 10%) and high dose( 20%) of SJZD containing serum upregulated TLR-2 and My D88 mRNA and protein expression,respectively. Conclusion: Sijunzi decoction can repair the injury of gastrointestinal mucosal barrier,the mechanism may be related to its effect on activating TLR-2 / My D88 signaling pathway,and promoting IEC-6 cell migration and proliferation.
Databáze:	MEDLINE
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