Laccase-Catalyzed Heterocoupling of Dihydroquercetin and p-Aminobenzoic Acid: Effect of the Reaction Product on Cultured Cells.

Autor: Khlupova ME; Research Center of Biotechnology, Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 119071, Russia., Morozova OV; Research Center of Biotechnology, Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 119071, Russia., Vasil'eva IS; Research Center of Biotechnology, Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 119071, Russia., Shumakovich GP; Research Center of Biotechnology, Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 119071, Russia., Pashintseva NV; Research Center of Biotechnology, Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 119071, Russia., Kovalev LI; Research Center of Biotechnology, Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 119071, Russia., Shishkin SS; Research Center of Biotechnology, Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 119071, Russia., Chertkov VA; Lomonosov Moscow State University, Faculty of Chemistry, Moscow, 119899, Russia. vchertkov@hotmail.com., Shestakova AK; State Research Institute of Chemistry and Technology of Organoelement Compounds, Moscow, 105118, Russia. alshestakova@yandex.ru., Kisin AV; State Research Institute of Chemistry and Technology of Organoelement Compounds, Moscow, 105118, Russia., Yaropolov AI; Research Center of Biotechnology, Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 119071, Russia. yaropolov@inbi.ras.ru.
Jazyk: angličtina
Zdroj: Biochemistry. Biokhimiia [Biochemistry (Mosc)] 2018 Aug; Vol. 83 (8), pp. 992-1001.
DOI: 10.1134/S0006297918080102
Abstrakt: Derivatization of the natural flavonoid dihydroquercetin with p-aminobenzoic acid was carried out in an ethyl acetate/citric buffer biphasic system using laccase from the fungus Trametes hirsuta. The main reaction product yield was ~68 mol %. The product was characterized by 1 H NMR, 13 C NMR, and liquid chromatography-mass spectroscopy, and its structure was elucidated. The reaction product affected viability of cultured human rhabdomyosarcoma cells (RD cell line) in a dose-dependent manner and, therefore, can be of interest to pharmaceutical industry.
Databáze: MEDLINE
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