Evolutionary history of Mo25 gene in plants, a component of RAM/MOR signaling network.

Autor: Bizotto FM; Computational, Structural and Molecular Biology Laboratory, Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, 5001 Avenida dos Estados, Santo André 09210-580, Brazil; Evolution and Diversity II Laboratory, Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Alameda da Universidade, s/n°, São Bernardo do Campo 09606-045, Brazil. Electronic address: fernanda.bizotto@ufabc.edu.br., Ceratti RS; Evolution and Diversity II Laboratory, Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Alameda da Universidade, s/n°, São Bernardo do Campo 09606-045, Brazil. Electronic address: rsceratti@ufabc.edu.br., Braz ASK; Computational, Structural and Molecular Biology Laboratory, Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, 5001 Avenida dos Estados, Santo André 09210-580, Brazil. Electronic address: antonio.braz@ufabc.edu.br., Masuda HP; Evolution and Diversity II Laboratory, Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Alameda da Universidade, s/n°, São Bernardo do Campo 09606-045, Brazil. Electronic address: hana.masuda@ufabc.edu.br.
Jazyk: angličtina
Zdroj: Mechanisms of development [Mech Dev] 2018 Oct; Vol. 153, pp. 64-73. Date of Electronic Publication: 2018 Sep 09.
DOI: 10.1016/j.mod.2018.09.001
Abstrakt: Change in cell morphogenesis is an important feature for proper development of eukaryotes. It is necessary for cell polarity and asymmetry and is essential for asymmetric cell division. RAM/MOR is a conserved signaling network that coordinates cell polarity determinants important for asymmetric cell division and cell polarity establishment. Mo25 is a scaffold protein that acts as a master regulator of the germinal center kinase (GCK) which triggers the downstream signaling of this network. Little is known about RAM/MOR network or Mo25 protein homologs in plants. Here, we provide a glimpse of the evolutionary gene history of Mo25 in green plants. Our data showed that a duplication of Mo25 occurred at the basis of land plants (Embryophyta), forming the groups Mo25A and Mo25B. Further duplication events occurred in other plant lineages and one subgroup of sequences seemed to be rapidly diverging. This subgroup contained an A. thaliana paralog (AtMo25-1) which lacks intron and is expressed in a similar fashion of retrogenes (i.e. low expression levels and narrow expression breadth), suggesting that this paralog was duplicated by retroposition. We also showed that all AtMo25 proteins are structurally similar to each other and to the human homolog, although differences in residues in the interface between human Mo25 and MST3 are observed in the A. thaliana homologs. Expression profile of AtMo25 homologs suggest that they are required at different developmental contexts, possibly interacting with different partners. Finally, we discuss whether Mo25 duplication in Embryophyta could be an evolutionary novelty important for the terrestrial environment conquest and whether the duplicated paralogs are undergoing neo- or subfunctionalization.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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