Photobiomodulation therapy improves multilineage differentiation of dental pulp stem cells in three-dimensional culture model.
| Autor: | Zaccara IM; Federal University of Rio Grande do Sul-UFRGS, School of Dentistry, Graduate Program, Department of, Brazil., Mestieri LB; Federal University of Rio Grande do Sul-UFRGS, School of Dentistry, Graduate Program, Department of, Brazil., Moreira MS; University Ibirapuera-UNIB, School of Dentistry, São Paulo, Brazil., Grecca FS; Federal University of Rio Grande do Sul-UFRGS, School of Dentistry, Graduate Program, Department of, Brazil., Martins MD; Federal University of Rio Grande do Sul-UFRGS, School of Dentistry, Graduate Program, Department of, Brazil., Kopper PMP; Federal University of Rio Grande do Sul-UFRGS, School of Dentistry, Graduate Program, Department of, Brazil. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of biomedical optics [J Biomed Opt] 2018 Sep; Vol. 23 (9), pp. 1-9. |
| DOI: | 10.1117/1.JBO.23.9.095001 |
| Abstrakt: | Photobiomodulation therapy (PBM) has shown positive effects on stem cell differentiation in monolayer cell culture model, but little is known about its effect on three-dimensional (3-D) agarose gel culture. This study evaluated the PBM effect of human dental pulp stem cells (hDPSCs) differentiation and phosphatase alkaline activity (ALP) using an agarose 3-D model under different nutritional conditions. hDPSCs were characterized and seeded on a 0.3% agarose gel layer with different media (osteogenic, adipogenic, chondrogenic) and were assigned into four groups: control 10% fetal bovine serum (FBS), control 5% FBS, PBM 10% FBS, and PBM 5% FBS. Irradiation was performed with continuous-wave InGaAlP laser, 660 nm, 100 mW, 3,3 J / cm2, spot size 0.3 cm2, 10 s of exposure time, and 1 J of energy per point with 6-h interval between sessions. All groups were evaluated at 7 and 14 days. ALP assay was performed to analyze the deposition of mineralized tissue. At 7 days, PBM 5% FBS group presented better stimulation in osteogenic and adipogenic differentiation compared with control. After 14 days, hDPSCs cultured in 3-D exhibited osteogenic, adipogenic, and chondrogenic differentiation; furthermore, compared to control, PBM significantly stimulated all differentiation processes (p < 0.05). It can be concluded that hDPSCs cultured in 3-D agarose associated to PBM could be a promising tool for tissue engineering applications. ((2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).) |
| Databáze: | MEDLINE |
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