Fluorescence-Based Measurements of the CRAC Channel Activity in Cell Populations.

Autor: Redondo PC; Department of Physiology (Cell Physiology Research Group), University of Extremadura, Cáceres, Spain., Berna-Erro A; Department of Physiology (Cell Physiology Research Group), University of Extremadura, Cáceres, Spain., Dionisio N; Department of Physiology (Cell Physiology Research Group), University of Extremadura, Cáceres, Spain., Rosado JA; Department of Physiology (Cell Physiology Research Group), University of Extremadura, Cáceres, Spain. jarosado@unex.es.
Jazyk: angličtina
Zdroj: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1843, pp. 69-82.
DOI: 10.1007/978-1-4939-8704-7_6
Abstrakt: Cytosolic Ca 2+ plays an important role in cellular biology, and since its identification as a second messenger, a number of techniques and methods to analyze the changes in cytosolic Ca 2+ concentration ([Ca 2+ ] c ) induced by physiological agonists have been developed. Changes in [Ca 2+ ] c might be determined in single cells or in cell populations. Measurement in single cells allows to determine changes in [Ca 2+ ] c at a subcellular level but often results in heterogeneous responses among cells. Determination of intracellular Ca 2+ mobilization at the cell population level reduces this heterogeneity and allows [Ca 2+ ] c measurements in small cells that load little amounts of indicator. Here, we describe the measurement of agonist-evoked changes in [Ca 2+ ] c associated with Ca 2+ influx in cell populations.
Databáze: MEDLINE
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