YraP Contributes to Cell Envelope Integrity and Virulence of Salmonella enterica Serovar Typhimurium.

Autor:	Morris FC; Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom., Wells TJ; Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom., Bryant JA; Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom., Schager AE; Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom., Sevastsyanovich YR; Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom., Squire DJP; Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom., Marshall J; Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom., Isom GL; Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom., Rooke J; Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom., Maderbocus R; Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom., Knowles TJ; Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom., Overduin M; Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom., Rossiter AE; Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom., Cunningham AF; Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom., Henderson IR; Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom i.r.henderson@bham.ac.uk.
Jazyk:	angličtina
Zdroj:	Infection and immunity [Infect Immun] 2018 Oct 25; Vol. 86 (11). Date of Electronic Publication: 2018 Oct 25 (Print Publication: 2018).
DOI:	10.1128/IAI.00829-17
Abstrakt:	Mutations in σ E -regulated lipoproteins have previously been shown to impact bacterial viability under conditions of stress and during in vivo infection. YraP is conserved across a number of Gram-negative pathogens, including Neisseria meningitidis , where the homolog is a component of the Bexsero meningococcal group B vaccine. Investigations using laboratory-adapted Escherichia coli K-12 have shown that yraP mutants have elevated sensitivity to a range of compounds, including detergents and normally ineffective antibiotics. In this study, we investigate the role of the outer membrane lipoprotein YraP in the pathogenesis of Salmonella enterica serovar Typhimurium. We show that mutations in S Typhimurium yraP result in a defective outer membrane barrier with elevated sensitivity to a range of compounds. This defect is associated with attenuated virulence in an oral infection model and during the early stages of systemic infection. We show that this attenuation is not a result of defects in lipopolysaccharide and O-antigen synthesis, changes in outer membrane protein levels, or the ability to adhere to and invade eukaryotic cell lines in vitro . (Copyright © 2018 American Society for Microbiology.)
Databáze:	MEDLINE
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