| Autor: |
Sherman LS; Division of Hematology/Oncology, Department of Medicine, New Jersey Medical School, Rutgers Biomedical and Health Sciences, Newark, NJ, USA., Condé-Green A; Division of Plastic Surgery, Department of Surgery, New Jersey Medical School, Rutgers School of Biomedical Health Science, Newark, NJ, USA., Kotamarti VS; New Jersey Medical School, Rutgers School of Biomedical Health Science, Newark, NJ, USA., Lee ES; Division of Plastic Surgery, Department of Surgery, New Jersey Medical School, Rutgers School of Biomedical Health Science, Newark, NJ, USA., Rameshwar P; Department of Medicine-Hematology/Oncology, Rutgers New Jersey Medical School, Newark, NJ, USA. rameshwa@njms.rutgers.edu. |
| Abstrakt: |
Mesenchymal stem cells (MSCs) are a population of multipotent cells that can be isolated from various adult and fetal tissues, including adipose tissue. These cells contain enormous clinical and basic research appeal due to their plasticity to differentiate into cells of all germ layers in vitro, cross allogeneic barriers in vivo, and suppress inflammation. Methods to isolate adipose-derived MSCs (ADSCs) primarily rely on enzymatic digestion of the adipose tissue using harsh enzymes such as collagenase. However, these harsh enzymes are expensive and can have detrimental effects on the ADSCs, including risks of using xenograft components in clinical application. This chapter focuses on methods of isolating ADSCs from adipose tissue without enzymatic digestion. |
