Acylated catalpol diglycoside ameliorates lipopolysaccharides-induced acute lung injury through inhibition of iNOS and TNF-α expression.

Autor: El-Awady MS; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Taibah University, Almadinah Almonawarah, Saudi Arabia.; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt., Said E; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt., Baraka HN; Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.; Department of Pharmacognosy, Faculty of Pharmacy, Delta University for Sciences and Technology, Gamasa, Egypt.
Jazyk: angličtina
Zdroj: Journal of biochemical and molecular toxicology [J Biochem Mol Toxicol] 2018 Nov; Vol. 32 (11), pp. e22214. Date of Electronic Publication: 2018 Sep 08.
DOI: 10.1002/jbt.22214
Abstrakt: Acute lung injury (ALI) is a universal cause of respiratory failure and death especially after sepsis. The current study evaluates the protective efficacy of acylated catalpol diglycoside (ACD), a plant iridoid glycoside, against lipopolysaccharides (LPS)-induced ALI in rats. ACD prevented LPS-induced elevations in total and differential cell counts and total protein content in bronchoalveolar lavage fluid, lung malondialdehyde content, and serum lactate dehydrogenase activity. Moreover, ACD significantly increased lung glutathione and superoxide dismutase and improved lung histopathology with significant reduction in lung tumor necrosis factor-α (TNF-α) content mediated mainly via inhibition of TNF-α messenger RNA (mRNA) expression. In addition, ACD significantly reduced lung total nitrate concentration content through downregulation of inducible nitric oxide synthase (iNOS) mRNA expression, negating the excessive undesired vasodilatation characteristic to sepsis. In conclusion, the reduction of oxidative stress, amelioration of inflammatory cytokines expression, and antagonism of sepsis associated-excessive vasodilatation are main contributors in ACD's protective effect in LPS-induced ALI in rats.
(© 2018 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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