Transcriptome of the Wistar audiogenic rat (WAR) strain following audiogenic seizures.

Autor: Damasceno S; Departamento de Biologia Geral, Universidade Federal de Minas Gerais, Pampulha, CEP 31270-901, Belo Horizonte, MG, Brazil., Menezes NB; Departamento de Biologia Geral, Universidade Federal de Minas Gerais, Pampulha, CEP 31270-901, Belo Horizonte, MG, Brazil., Rocha CS; Departamento de Genética Médica, Faculdade de Ciências Medicas, Universidade de Campinas, Cidade Universitária Zeferino Vaz, CEP 13083-887, Campinas, SP, Brazil., Matos AHB; Departamento de Genética Médica, Faculdade de Ciências Medicas, Universidade de Campinas, Cidade Universitária Zeferino Vaz, CEP 13083-887, Campinas, SP, Brazil., Vieira AS; Departamento de Genética Médica, Faculdade de Ciências Medicas, Universidade de Campinas, Cidade Universitária Zeferino Vaz, CEP 13083-887, Campinas, SP, Brazil., Moraes MFD; Departamento de Fisiologia e Biofísica, Universidade Federal de Minas Gerais, Pampulha, CEP 31270-901, Belo Horizonte, MG, Brazil., Martins AS; Departamento de Fisiologia e Biofísica, Universidade Federal de Minas Gerais, Pampulha, CEP 31270-901, Belo Horizonte, MG, Brazil., Lopes-Cendes I; Departamento de Genética Médica, Faculdade de Ciências Medicas, Universidade de Campinas, Cidade Universitária Zeferino Vaz, CEP 13083-887, Campinas, SP, Brazil., Godard ALB; Departamento de Biologia Geral, Universidade Federal de Minas Gerais, Pampulha, CEP 31270-901, Belo Horizonte, MG, Brazil. Electronic address: brunialt@ufmg.br.
Jazyk: angličtina
Zdroj: Epilepsy research [Epilepsy Res] 2018 Nov; Vol. 147, pp. 22-31. Date of Electronic Publication: 2018 Aug 29.
DOI: 10.1016/j.eplepsyres.2018.08.010
Abstrakt: The Wistar Audiogenic Rat (WAR) is a model whose rats are predisposed to develop seizures following acoustic stimulation. We aimed to establish the transcriptional profile of the WAR model, searching for genes that help in understanding the molecular mechanisms involved in the predisposition and seizures expression of this strain. RNA-Seq of the corpora quadrigemina of WAR and Wistar rats subjected to acoustic stimulation revealed 64 genes differentially regulated in WAR. We validated twelve of these genes by qPCR in stimulated and naive (non-stimulated) WAR and Wistar rats. Among these, Acsm3 was upregulated in WAR in comparison with both control groups. In contrast, Gpr126 and Rtel1 were downregulated in naive and stimulated WAR rats in comparison with the Wistar controls. Qdpr was upregulated only in stimulated WAR rats that exhibited audiogenic seizures. Our data show that there are genes with differential intrinsic regulation in the WAR model and that seizures can alter gene regulation. We identified new genes that might be involved in the epileptic phenotype and comorbidities of the WAR model.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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