| Autor: |
Mladenović B; a Clinic for Gastroenterology, Clinical Center Niš, 18000 Niš, Serbia.; b Department of Internal Medicine, Faculty of Medicine, University of Niš, Zorana Ðinđića 81, 18000 Niš, Serbia., Mladenović N; c Institute for Cardiovascular Diseases Sremska Kamenica, Put doktora Goldmana 4, 21208 Sremska Kamenica, Serbia., Brzački V; a Clinic for Gastroenterology, Clinical Center Niš, 18000 Niš, Serbia.; b Department of Internal Medicine, Faculty of Medicine, University of Niš, Zorana Ðinđića 81, 18000 Niš, Serbia., Petrović N; c Institute for Cardiovascular Diseases Sremska Kamenica, Put doktora Goldmana 4, 21208 Sremska Kamenica, Serbia., Kamenov A; d Clinic for Cardiovascular and Transplantation Surgery, Clinical Center Niš, 18000 Niš, Serbia., Golubović M; e Clinic for Anesthesiology and Intensive Therapy, Department for Cardiosurgery, Clinical Center Nis, 18000 Niš, Serbia., Ničković V; f Clinical-Hospital Center, Pristina, Gracanica, Serbia., Stojanović NM; g Faculty of Medicine, University of Niš, Zorana Ðinđića 81, 18000 Niš, Serbia., Sokolović DT; h Department of Biochemistry, Faculty of Medicine, University of Niš, Zorana Ðinđića 81, 18000 Niš, Serbia. |
| Abstrakt: |
Rat bile duct ligation (BDL) represents a useful method that mimics obstructive extrahepatic cholestasis, which is known to be a frequent disorder in humans. Polyamines (putrescine, spermidine, and spermine) are one of the key molecules regulating cell proliferation and differentiation. This work aimed to evaluate the potential beneficial properties of putrescine in rat BDL model by studying several biochemical parameters reflecting liver function and polyamine metabolism. Rats that were subjected to BDL were injected with putrescine (150 mg/kg) for 9 days, while in parallel another group with BDL remained untreated. Two control groups were included as well, sham-opened and putrescine-treated group. The following plasma parameters: ALT, AST, γ-GT, ALP, bilirubin, bile acids, as well as liver malondialdehyde and polyamine concentration and the activity of enzymes involved in polyamine metabolism were studied. After BDL, significant alterations in plasma biochemical parameters occurred, where a 9-day putrescine treatment significantly alleviated liver function deterioration. Putrescine also increased liver polyamines' concentrations and polyamine and diamine oxidase activities in rats submitted to BDL. Our results demonstrated, for the first time, that putrescine plays an important role in preserving liver tissue function in rats with experimentally induced cholestasis. |
