A Modular Approach to Sensitized Two-Photon Patterning of Photodegradable Hydrogels.

Autor: Lunzer M; Institute of Materials Science and Technology, TU Wien, Getreidemarkt 9/308, 1060, Vienna, Austria.; Institute of Applied Synthetic Chemistry, TU Wien, Getreidemarkt 9/163-MC, 1060, Vienna, Austria.; Austrian Cluster for Tissue Regeneration, Austria., Shi L; Department of Chemistry-Ångström Laboratory, Uppsala University, Lägerhyddsvägen 1, 751 21, Uppsala, Sweden., Andriotis OG; Institute of Lightweight Design and Structural Biomechanics, TU Wien, Getreidemarkt 9/317, 1060, Vienna, Austria.; Austrian Cluster for Tissue Regeneration, Austria., Gruber P; Institute of Materials Science and Technology, TU Wien, Getreidemarkt 9/308, 1060, Vienna, Austria.; Austrian Cluster for Tissue Regeneration, Austria., Markovic M; Institute of Materials Science and Technology, TU Wien, Getreidemarkt 9/308, 1060, Vienna, Austria.; Austrian Cluster for Tissue Regeneration, Austria., Thurner PJ; Institute of Lightweight Design and Structural Biomechanics, TU Wien, Getreidemarkt 9/317, 1060, Vienna, Austria.; Austrian Cluster for Tissue Regeneration, Austria., Ossipov D; Department of Chemistry-Ångström Laboratory, Uppsala University, Lägerhyddsvägen 1, 751 21, Uppsala, Sweden.; Department of Biosciences and Nutrition, Karolinska Institutet, Novum, 141 83 Huddinge, Stockholm, Sweden., Liska R; Institute of Applied Synthetic Chemistry, TU Wien, Getreidemarkt 9/163-MC, 1060, Vienna, Austria.; Austrian Cluster for Tissue Regeneration, Austria., Ovsianikov A; Institute of Materials Science and Technology, TU Wien, Getreidemarkt 9/308, 1060, Vienna, Austria.; Austrian Cluster for Tissue Regeneration, Austria.
Jazyk: angličtina
Zdroj: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2018 Nov 12; Vol. 57 (46), pp. 15122-15127. Date of Electronic Publication: 2018 Oct 18.
DOI: 10.1002/anie.201808908
Abstrakt: Photodegradable hydrogels have emerged as useful platforms for research on cell function, tissue engineering, and cell delivery as their physical and chemical properties can be dynamically controlled by the use of light. The photo-induced degradation of such hydrogel systems is commonly based on the integration of photolabile o-nitrobenzyl derivatives to the hydrogel backbone, because such linkers can be cleaved by means of one- and two-photon absorption. Herein we describe a cytocompatible click-based hydrogel containing o-nitrobenzyl ester linkages between a hyaluronic acid backbone, which is photodegradable in the presence of cells. It is demonstrated for the first time that by using a cyclic benzylidene ketone-based small molecule as photosensitizer the efficiency of the two-photon degradation process can be improved significantly. Biocompatibility of both the improved two-photon micropatterning process as well as the hydrogel itself is confirmed by cell culture studies.
(© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
Externí odkaz: