The Challenges of DNA Extraction in Different Assorted Food Matrices: A Review.

Autor: Sajali N; School of Engineering and Technology, University College of Technology Sarawak, 868 Persiaran Brooke, 96000, Sibu, Sarawak, Malaysia.; Halal Products Research Institute, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia., Wong SC; Department of Basic Science and Engineering, Faculty of Agriculture and Food Sciences, Universiti Putra Malaysia Bintulu Sarawak Campus, P.O. Box 396, Nyabau Road, 97008, Bintulu, Sarawak, Malaysia., Hanapi UK; Halal Products Research Institute, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia., Abu Bakar Jamaluddin S; Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, Selangor Darul Ehsan, Malaysia., Tasrip NA; Halal Products Research Institute, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia., Mohd Desa MN; Halal Products Research Institute, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia.; Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, Selangor Darul Ehsan, Malaysia.
Jazyk: angličtina
Zdroj: Journal of food science [J Food Sci] 2018 Oct; Vol. 83 (10), pp. 2409-2414. Date of Electronic Publication: 2018 Sep 05.
DOI: 10.1111/1750-3841.14338
Abstrakt: High-quality DNA extracts are imperative for downstream applications in molecular identification. Most processed food products undergo heat treatments causing DNA degradation, which hampers application of DNA-based techniques for food authentication. Moreover, the presence of inhibitors in processed food products is also problematic, as inhibitors can impede the process of obtaining high qualities and quantities of DNA. Various approaches in DNA extraction and factors in structure and texture of various food matrices affecting DNA extraction are explained in this review.
(© 2018 Institute of Food Technologists®.)
Databáze: MEDLINE
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