Secondary Photocrosslinking of Click Hydrogels To Probe Myoblast Mechanotransduction in Three Dimensions.

Autor: Brown TE; Department of Chemical and Biological Engineering , University of Colorado , Boulder , Colorado 80303 , United States.; BioFrontiers Institute , University of Colorado , Boulder , Colorado 80303 , United States., Silver JS; Department of Chemical and Biological Engineering , University of Colorado , Boulder , Colorado 80303 , United States.; BioFrontiers Institute , University of Colorado , Boulder , Colorado 80303 , United States., Worrell BT; Department of Chemical and Biological Engineering , University of Colorado , Boulder , Colorado 80303 , United States., Marozas IA; Department of Chemical and Biological Engineering , University of Colorado , Boulder , Colorado 80303 , United States.; BioFrontiers Institute , University of Colorado , Boulder , Colorado 80303 , United States., Yavitt FM; Department of Chemical and Biological Engineering , University of Colorado , Boulder , Colorado 80303 , United States.; BioFrontiers Institute , University of Colorado , Boulder , Colorado 80303 , United States., Günay KA; Department of Chemical and Biological Engineering , University of Colorado , Boulder , Colorado 80303 , United States.; BioFrontiers Institute , University of Colorado , Boulder , Colorado 80303 , United States., Bowman CN; Department of Chemical and Biological Engineering , University of Colorado , Boulder , Colorado 80303 , United States.; BioFrontiers Institute , University of Colorado , Boulder , Colorado 80303 , United States.; Department of Materials Science and Engineering , University of Colorado , Boulder , Colorado 80303 , United States., Anseth KS; Department of Chemical and Biological Engineering , University of Colorado , Boulder , Colorado 80303 , United States.; BioFrontiers Institute , University of Colorado , Boulder , Colorado 80303 , United States.
Jazyk: angličtina
Zdroj: Journal of the American Chemical Society [J Am Chem Soc] 2018 Sep 19; Vol. 140 (37), pp. 11585-11588. Date of Electronic Publication: 2018 Sep 10.
DOI: 10.1021/jacs.8b07551
Abstrakt: Muscle cells sense the mechanical properties of their microenvironment, and these properties can change in response to injury or disease. Hydrogels with dynamic material properties can be used to study the effect of such varying mechanical signals. Here, we report the ability of azadibenzocyclooctyne to undergo a cytocompatible, photoinitiated crosslinking reaction. This reaction is exploited as a strategy for on-demand stiffening of three-dimensional cell scaffolds formed through an initial strain-promoted azide-alkyne cycloaddition. Myoblasts encapsulated in these networks respond to increased matrix stiffness through decreased cell spreading and nuclear localization of Yes-associated protein 1 (YAP). However, when the photocrosslinking reaction is delayed to allow cell spreading, elongated myoblasts display increased YAP nuclear localization.
Databáze: MEDLINE
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