In situ characterization of change in superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25.

Autor: Liu LZ; School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China., Nie ZY; School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China; Key Lab of Biometallurgy of the Ministry of Education of China, Central South University, Changsha, 410083, China. Electronic address: zynie@csu.edu.cn., Yang Y; CSIRO Mineral Resources, Clayton South, Vic, 3169, Australia., Pan X; School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China., Xia X; School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China., Zhou YH; School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China., Xia JL; School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China; Key Lab of Biometallurgy of the Ministry of Education of China, Central South University, Changsha, 410083, China. Electronic address: jlxia@csu.edu.cn., Zhang LJ; Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201204, China., Zhen XJ; Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201204, China., Yang HY; School of Metallurgy, Northeastern University, Shenyang, 110819, China.
Jazyk: angličtina
Zdroj: Research in microbiology [Res Microbiol] 2018 Dec; Vol. 169 (10), pp. 590-597. Date of Electronic Publication: 2018 Sep 01.
DOI: 10.1016/j.resmic.2018.08.003
Abstrakt: For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS 2 , CuFeS 2 , S 0 , FeSO 4 ). The atomic force microscopy (AFM) morphology scanning showed that the strain acclimated with different energy substrates varied a lot in EPS amount. XANES results showed clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (CO, CO and CN). The chalcopyrite- and pyrite-acclimated cells contained higher proportion of proteins but less proportion of polysaccharides than the S 0 -acclimated cells. The FeSO 4 -acclimated cells contained the highest proportion of proteins, while the S 0 -acclimated cells contained more lipids and polysaccharides. The results of linear-combination and peak fitting of the K-edge XANES for the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there are significant differences in the values. These differences are caused by the inconsistencies of measurement depth between XANES and the latter two characterization methods.
(Copyright © 2018 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: