| Autor: |
Gautier C; PEX Biotechnologie Chimie & Biologie, Institut de Recherches Servier, Croissy sur Seine, France.; Institut des Neurosciences Cellulaires et Intégratives, Strasbourg, France., Bothorel B; Institut des Neurosciences Cellulaires et Intégratives, Strasbourg, France., Ciocca D; Institut des Neurosciences Cellulaires et Intégratives, Strasbourg, France., Valour D; PEX Méthodologie et Valorisation des Données, Institut de Recherches Internationales Servier, Suresnes, France., Gaudeau A; PEX Biotechnologie Chimie & Biologie, Institut de Recherches Servier, Croissy sur Seine, France., Dupré C; PEX Biotechnologie Chimie & Biologie, Institut de Recherches Servier, Croissy sur Seine, France., Lizzo G; PEX Biotechnologie Chimie & Biologie, Institut de Recherches Servier, Croissy sur Seine, France., Brasseur C; PEX Biotechnologie Chimie & Biologie, Institut de Recherches Servier, Croissy sur Seine, France., Riest-Fery I; PEX Biotechnologie Chimie & Biologie, Institut de Recherches Servier, Croissy sur Seine, France., Stephan JP; PEX Biotechnologie Chimie & Biologie, Institut de Recherches Servier, Croissy sur Seine, France., Nosjean O; PEX Biotechnologie Chimie & Biologie, Institut de Recherches Servier, Croissy sur Seine, France.; Institut de Recherches Internationales Servier, Suresnes, France., Boutin JA; PEX Biotechnologie Chimie & Biologie, Institut de Recherches Servier, Croissy sur Seine, France. jean.boutin@servier.com.; Institut de Recherches Internationales Servier, Suresnes, France. jean.boutin@servier.com., Guénin SP; PEX Biotechnologie Chimie & Biologie, Institut de Recherches Servier, Croissy sur Seine, France., Simonneaux V; Institut des Neurosciences Cellulaires et Intégratives, Strasbourg, France. simonneaux@inci-cnrs.unistra.fr. |
| Abstrakt: |
Hibernation is an exceptional physiological response to a hostile environment, characterized by a seasonal period of torpor cycles involving dramatic reductions of body temperature and metabolism, and arousal back to normothermia. As the mechanisms regulating hibernation are still poorly understood, here we analysed the expression of genes involved in energy homeostasis, torpor regulation, and daily or seasonal timing using digital droplet PCR in various central and peripheral tissues sampled at different stages of torpor/arousal cycles in the European hamster. During torpor, the hypothalamus exhibited strongly down-regulated gene expression, suggesting that hypothalamic functions were reduced during this period of low metabolic activity. During both torpor and arousal, many structures (notably the brown adipose tissue) exhibited altered expression of deiodinases, potentially leading to reduced tissular triiodothyronine availability. During the arousal phase, all analysed tissues showed increased expression of the core clock genes Per1 and Per2. Overall, our data indicated that the hypothalamus and brown adipose tissue were the tissues most affected during the torpor/arousal cycle, and that clock genes may play critical roles in resetting the body's clocks at the beginning of the active period. |
