| Autor: |
Choi Y; Department of Electrical and Computer Engineering, Seoul National University., Choi H; Department of Electrical and Computer Engineering, Seoul National University., Lee AC; Interdisciplinary Program for Bioengineering, Seoul National University., Kwon S; Department of Electrical and Computer Engineering, Seoul National University; Interdisciplinary Program for Bioengineering, Seoul National University; Institute of Entrepreneurial Bio Convergence, Seoul National University; Seoul National University Hospital Biomedical Research Institute, Seoul National University Hospital; skwon@snu.ac.kr. |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of visualized experiments : JoVE [J Vis Exp] 2018 Aug 15 (138). Date of Electronic Publication: 2018 Aug 15. |
| DOI: |
10.3791/58364 |
| Abstrakt: |
DNA nanostructure-based mechanical systems or DNA nanomachines, which produce complex nanoscale motion in 2D and 3D in the nanometer to ångström resolution, show great potential in various fields of nanotechnology such as the molecular reactors, drug delivery, and nanoplasmonic systems. The reconfigurable DNA accordion rack, which can collectively manipulate a 2D or 3D nanoscale network of elements, in multiple stages in response to the DNA inputs, is described. The platform has potential to increase the number of elements that DNA nanomachines can control from a few elements to a network scale with multiple stages of reconfiguration. In this protocol, we describe the entire experimental process of the reconfigurable DNA accordion rack of 6 by 6 meshes. The protocol includes a design rule and simulation procedure of the structures and a wet-lab experiment for synthesis and reconfiguration. In addition, analysis of the structure using TEM (transmission electron microscopy) and FRET (fluorescence resonance energy transfer) is included in the protocol. The novel design and simulation methods covered in this protocol will assist researchers to use the DNA accordion rack for further applications. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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