| Autor: |
Magagnin S; Biologa Nutrizionista, Casatenovo, Milan, Italy., Werner A; Institute for Cell and Molecular Biosciences, Epithelial Research Group, Newcastle University, Newcastle, UK. andreas.werner@ncl.ac.uk. |
| Jazyk: |
angličtina |
| Zdroj: |
Pflugers Archiv : European journal of physiology [Pflugers Arch] 2019 Jan; Vol. 471 (1), pp. 7-14. Date of Electronic Publication: 2018 Sep 03. |
| DOI: |
10.1007/s00424-018-2198-9 |
| Abstrakt: |
In the pre-genomic era, the cloning of a cDNA represented a significant achievement, particularly if the gene of interest encoded a membrane protein. At the time, molecular probes such as partial peptide sequences, suitable nucleic acid sequences, or antibodies were unavailable for most proteins and the "sodium-phosphate transporter" was no exception. In contrast, brush-border membrane vesicles and epithelial cell culture experiments had established a reliable set of functional hallmarks that described Na-dependent phosphate transport activity in some detail. Moreover, aspects of hormonal regulation of phosphate homeostasis could be recapitulated in these model systems. Expression cloning elegantly combined functional protein expression in Xenopus laevis oocytes with molecular biology to overcome the lack of molecular probes. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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