A Map of Toll-like Receptor Expression in the Intestinal Epithelium Reveals Distinct Spatial, Cell Type-Specific, and Temporal Patterns.

Autor:	Price AE; Division of Immunology & Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA., Shamardani K; Division of Immunology & Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA., Lugo KA; Division of Immunology & Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA., Deguine J; Division of Immunology & Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA., Roberts AW; Division of Immunology & Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA., Lee BL; Division of Immunology & Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA., Barton GM; Division of Immunology & Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA. Electronic address: barton@berkeley.edu.
Jazyk:	angličtina
Zdroj:	Immunity [Immunity] 2018 Sep 18; Vol. 49 (3), pp. 560-575.e6. Date of Electronic Publication: 2018 Aug 28.
DOI:	10.1016/j.immuni.2018.07.016
Abstrakt:	Signaling by Toll-like receptors (TLRs) on intestinal epithelial cells (IECs) is critical for intestinal homeostasis. To visualize epithelial expression of individual TLRs in vivo, we generated five strains of reporter mice. These mice revealed that TLR expression varied dramatically along the length of the intestine. Indeed, small intestine (SI) IECs expressed low levels of multiple TLRs that were highly expressed by colonic IECs. TLR5 expression was restricted to Paneth cells in the SI epithelium. Intestinal organoid experiments revealed that TLR signaling in Paneth cells or colonic IECs induced a core set of host defense genes, but this set did not include antimicrobial peptides, which instead were induced indirectly by inflammatory cytokines. This comprehensive blueprint of TLR expression and function in IECs reveals unexpected diversity in the responsiveness of IECs to microbial stimuli, and together with the associated reporter strains, provides a resource for further study of innate immunity. (Copyright © 2018 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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