Protective effect of inactivated blastoconidia in keratinocytes and human reconstituted epithelium against C. albicans infection.

Autor:	Alburquenque C; Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina Universidad de Chile. Santiago de Chile.; Escuela de Tecnología Médica, Facultad de Ciencias, Universidad Mayor, Santiago de Chile., Amaro J; Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina Universidad de Chile. Santiago de Chile., Fuentes M; Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina Universidad de Chile. Santiago de Chile., Falconer MA; Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina Universidad de Chile. Santiago de Chile., Moreno C; Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina Universidad de Chile. Santiago de Chile., Covarrubias C; Facultad de Odontología Universidad de Chile. Laboratorio de Nanomateriales. Santiago, Chile., Pinto C; Unidad de Anatomía Patológica Clínica Dávila, Santiago, Chile., Rodas PI; Laboratorio de Microbiología Médica y Patogénesis Bacteriana, Facultad de Medicina, Universidad Andrés Bello, Concepción, Chile., Bucarey SA; Centro Biotecnológico Biovetec, Departamento de Ciencias Biológicas Animales. Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago de Chile., Hermosilla G; Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina Universidad de Chile. Santiago de Chile., Magne F; Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina Universidad de Chile. Santiago de Chile., Tapia CV; Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina Universidad de Chile. Santiago de Chile.; Laboratorio Clínica Dávila, Santiago, Chile.
Jazyk:	angličtina
Zdroj:	Medical mycology [Med Mycol] 2019 Jun 01; Vol. 57 (4), pp. 457-467.
DOI:	10.1093/mmy/myy068
Abstrakt:	Candida albicans is commensal yeast that colonizes skin and mucosa; however, it can become an opportunist pathogen by changing from blastoconidia (commensal form) into hypha (pathogenic form). Each form activates a different cytokines response in epithelial cells. Little is known about the commensal role of C. albicans in the innate immunity. This work studied whether stimulation with C. albicans blastoconidia induces protection in keratinocytes and/or in a reconstituted human epithelium (RHE) infected with C. albicans. For this, inactivated C. albicans blastoconidia was used to stimulate keratinocytes and RHE prior to infection with C. albicans. Blastoconidia induced different cytokine expression profiles; in the case of RHE it decreased interleukin (IL)-1β and IL-10 and increased IL-8, tumor necrosis factor α (TNF-α), and interferon γ (IFN-γ). A significant increase in the expression of human β-defensins (HBD) 2 and HBD3 was observed in blastoconidia stimulated keratinocytes and RHE, associated with impaired growth and viability of C. albicans. Additionally, blastoconidia stimulation decreased the expression of virulence factors in C. albicans that are associated with filamentation (EFG1, CPH1 and NRG1), adhesion (ALS5), and invasion (SAP2). Blastoconidia stimulated RHE was significantly less damaged by C. albicans invasion. These results show that the commensal form of C. albicans would exert a protective effect against self-infection. (© The Author(s) 2018. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.)
Databáze:	MEDLINE
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