| Autor: |
Meneses-Sagrero SE; Posgrado de Maestría en Ciencias de la Salud, Universidad de Sonora, Blvd. Colosio y Reforma S/N, Col. Centro, C.P. 83000, Hermosillo, Sonora, Mexico., Navarro-Navarro M; Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Blvd. Encinas y Rosales S/N, Col. Centro, C.P. 83000, Hermosillo, Sonora, Mexico., Ruiz-Bustos E; Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Blvd. Encinas y Rosales S/N, Col. Centro, C.P. 83000, Hermosillo, Sonora, Mexico., Del-Toro-Sánchez CL; Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Blvd. Encinas y Rosales S/N, Col. Centro, C.P. 83000, Hermosillo, Sonora, Mexico., Jiménez-Estrada M; Instituto de Química, Departamento de Productos Naturales, Universidad Nacional Autónoma de México, Cd. Universitaria, Cd. de México, Mexico., Robles-Zepeda RE; Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Blvd. Encinas y Rosales S/N, Col. Centro, C.P. 83000, Hermosillo, Sonora, Mexico. |
| Abstrakt: |
Cancer is the major cause of death in the world, representing a significant public health problem. Plants have been shown as a great source of secondary metabolites with anticancer activity. The aim of this work was evaluated the antiproliferative activity of the methanolic extracts, chemical fractions and the compound spinasterol isolated of medicinal plant Stegnosperma halimifolium . The methanolic extracts of stem, leaf and stem/leaf was obtained by maceration. The methanolic extract of stem was purified by successive extractions with solvents as n -hexane, ethyl acetate and ethanol. The n -hexane fraction was separated by column chromatographic and monitored by thin layer chromatographic. The compound spinasterol was characterized by 1 H NMR, 13 C NMR and Mass Spectrometry. Methanolic extracts, chemical, chromatographic fractions and spinasterol was evaluated against RAW 264.7, M12.C3.F6, PC-3 , LS-180, A549 and HeLa cancer cell lines by the standardized method MTT for determinate the antiproliferative activity. Methanolic extract of stem shown the better antiproliferative activity against the murine macrophage cancer cell line RAW 264.7. n -Hexane chemical fraction shown antiproliferative activity against human alveolar cancer cell line A549 and RAW 264.7. Was isolated and characterized a compound by NMR 1 H and 13 C, revealing the presence of sterol spinasterol. Spinasterol shown to have antiproliferative activity against cervical cancer cell line HeLa and RAW 264.7, indicating that spinasterol can be a responsible compound of antiproliferative activity found in the methanolic extract of Stegnosperma halimifolium . |
