Evaluation of skimmed milk flocculation method for virus recovery from tomatoes.

Autor:	Melgaço FG; Instituto Oswaldo Cruz - Fiocruz, Laboratório de Virologia Comparativa e Ambiental, Rio de Janeiro, RJ, Brazil. Electronic address: fabianagilmelgaco@gmail.com., Corrêa AA; Universidade Federal Fluminense, Laboratório de Virologia, Niterói, RJ, Brazil., Ganime AC; Instituto Oswaldo Cruz - Fiocruz, Laboratório de Virologia Comparativa e Ambiental, Rio de Janeiro, RJ, Brazil., Brandão MLL; Instituto Oswaldo Cruz - Fiocruz, Instituto Nacional de Controle de Qualidade em Saúde, Departamento de Imunologia, Laboratório de Vacinas Virais, Biofarmacêuticos e Cultura Celular, Rio de Janeiro, RJ, Brazil., Medeiros VM; Instituto Oswaldo Cruz - Fiocruz, Instituto Nacional de Controle de Qualidade em Saúde, Departamento de Microbiologia, Setor de Alimentos, Laboratório de Alimentos e Saneantes, Rio de Janeiro, RJ, Brazil., Rosas CO; Instituto Oswaldo Cruz - Fiocruz, Instituto Nacional de Controle de Qualidade em Saúde, Departamento de Microbiologia, Setor de Alimentos, Laboratório de Alimentos e Saneantes, Rio de Janeiro, RJ, Brazil., Lopes SMDR; Instituto Oswaldo Cruz - Fiocruz, Instituto Nacional de Controle de Qualidade em Saúde, Departamento de Microbiologia, Setor de Alimentos, Laboratório de Alimentos e Saneantes, Rio de Janeiro, RJ, Brazil., Miagostovich MP; Instituto Oswaldo Cruz - Fiocruz, Laboratório de Virologia Comparativa e Ambiental, Rio de Janeiro, RJ, Brazil.
Jazyk:	angličtina
Zdroj:	Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2018 Nov; Vol. 49 Suppl 1, pp. 34-39. Date of Electronic Publication: 2018 Aug 16.
DOI:	10.1016/j.bjm.2018.04.014
Abstrakt:	This study aimed to evaluate the elution-concentration methodology based on skimmed milk flocculation from three varieties of tomatoes (Solanum lycopersicum L. [globe], Solanum lycopersicum var. cerasiforme [cherry] and hybrid cocktail [grape tomato]) for further monitoring of field samples. Spiking experiments were performed to determine the success rate and efficiency recovery of human norovirus (NoV) genogroup II, norovirus murine-1 (MNV-1) used as sample process control virus and human adenovirus (HAdV). Mean values of 18.8%, 2.8% and 44.0% were observed for NoV GII, MNV-1 and HAdV, respectively with differences according to the types of tomatoes, with lower efficiency for cherry tomatoes. Analysis of 90 samples, obtained at commercial establishments in the metropolitan region of Rio de Janeiro State, revealed 4.5% positivity for HAdV. Bacterial analysis was also performed with no detection of Salmonella spp., L. monocytogenes and fecal coliforms. Data demonstrated that the skimmed milk flocculation method is suitable for recovering HAdV from tomatoes and highlights the need for considering investigation in order to improve food safety. (Copyright © 2018 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. All rights reserved.)
Databáze:	MEDLINE
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