| Autor: |
Salvadori C; 1 Dipartimento di Scienze Veterinarie, Università di Pisa, Viale delle Piagge, 2, 56124 Pisa, Italia., Formenti N; 2 Dipartimento di Medicina Veterinaria, Università di Milano, Via Celoria, 10, 20133 Milano, Italia., Trogu T; 2 Dipartimento di Medicina Veterinaria, Università di Milano, Via Celoria, 10, 20133 Milano, Italia., Lanfranchi P; 2 Dipartimento di Medicina Veterinaria, Università di Milano, Via Celoria, 10, 20133 Milano, Italia., Rossi L; 3 Dipartimento di Scienze Veterinarie, Università degli Studi di Torino, Largo Paolo Braccini 2, 10095 Grugliasco (Torino), Italia., Citterio C; 4 Istituto Zooprofilattico Sperimentale delle Venezie, Sezione di Belluno, Via M. Cappellari, 44-32100 Belluno (Belluno), Italia., Obber F; 4 Istituto Zooprofilattico Sperimentale delle Venezie, Sezione di Belluno, Via M. Cappellari, 44-32100 Belluno (Belluno), Italia., Poli A; 1 Dipartimento di Scienze Veterinarie, Università di Pisa, Viale delle Piagge, 2, 56124 Pisa, Italia. |
| Abstrakt: |
Trombiculosis has been reported in some wild ruminant species. We investigated the occurrence of trombiculosis in the northern chamois ( Rupicapra rupicapra rupicapra) in the Western Italian Alps, and we describe the related histopathologic changes. Superficial scrapes and skin samples were taken from 191 chamois from the Lombardy and Piedmont regions during the hunting season in September-December 2015. Numbers of eosinophils, mast cells, cluster of differentiation (CD)3-, CD79α-, CD68-positive cells were determined on immunohistochemically stained skin sections using a semiautomatic analysis system. Forty (20.9, 40/191) chamois were positive for trombiculid larvae on skin scrapings. Of the positive animals, 15 were from Lombardy and 25 from Piedmont, with similar prevalences. Macroscopic lesions were light with involvement of body regions that had contact with the ground, especially head (pinnae and areas around eyes and mouth) and limbs, where stylostome was easily formed due to thin skin. Histologically, trombiculosis caused a focal moderate dermatitis with epidermal necrosis, thin crusts, and hyperkeratosis. Inflammatory infiltrates were suggestive of a granulomatous reaction centered on a stylostome, formed by mite saliva and necrotic host tissue debris. However, we detected some difference in cutaneous immune response with some chamois showing a prevalent T-cell response and others having an increased B-cell count associated with a higher number of eosinophils, mast cells and a lower number of T cells. |
