Association of Elevated Serum GM-CSF, IFN- γ , IL-4, and TNF- α Concentration with Tobacco Smoke Induced Chronic Obstructive Pulmonary Disease in a South Indian Population.

Autor: Mitra A; SRM Research Institute, SRM University, Chennai 603203, India., Vishweswaraiah S; SRM Research Institute, SRM University, Chennai 603203, India., Thimraj TA; SRM Research Institute, SRM University, Chennai 603203, India., Maheswarappa M; JSS Medical College and Hospital, JSS University, Department of Pulmonary Medicine, Mysuru, India., Krishnarao CS; JSS Medical College and Hospital, JSS University, Department of Pulmonary Medicine, Mysuru, India., Sundararaja Lokesh K; JSS Medical College and Hospital, JSS University, Department of Pulmonary Medicine, Mysuru, India., Biligere Siddaiah J; JSS Medical College and Hospital, JSS University, Department of Pulmonary Medicine, Mysuru, India., Ganguly K; SRM Research Institute, SRM University, Chennai 603203, India.; Work Environment Toxicology, Institute of Environmental Medicine, Karolinska Institutet, Box 287, SE-171 77 Stockholm, Sweden., Anand MP; JSS Medical College and Hospital, JSS University, Department of Pulmonary Medicine, Mysuru, India.
Jazyk: angličtina
Zdroj: International journal of inflammation [Int J Inflam] 2018 Aug 01; Vol. 2018, pp. 2027856. Date of Electronic Publication: 2018 Aug 01 (Print Publication: 2018).
DOI: 10.1155/2018/2027856
Abstrakt: Background: Chronic obstructive pulmonary disease (COPD) is a devastating condition with limited pharmacotherapeutic options and exceptionally high public-health burden globally as well as in India. Tobacco smoking is the primary cause for COPD among men in India. Systemic inflammation involving altered regulation of cytokines controlling the host defense mechanism is a hallmark of COPD pathogenesis. However, biomarker discovery studies are limited among Indian COPD patients.
Methods: We assessed the serum concentrations [median (25th-75th percentile) pg/ml] of interleukin ( IL )-2,4,6,8,10, granulocyte macrophage colony stimulating factor ( GM-CSF ), interferon gamma ( IFN- γ ), and tumor necrosis factor alpha ( TNF- α ) using a multiplexed immunoassay. Our study cohort consisted of 30 tobacco smokers with COPD ( TS COPD ) and 20 tobacco smokers without COPD ( TS CONTROL ) from South India. The study population was matched for age, sex (male), and tobacco consumption (pack-years). COPD was diagnosed according to the global initiative for chronic obstructive lung disease (GOLD) criteria of persistent airflow obstruction determined by the ratio of postbronchodilator forced expiratory volume in 1 second/forced vital capacity (FEV 1 /FVC) of <0.7. A validated structured questionnaire-based survey [Burden of Obstructive Lung Disease (BOLD) study] and spirometry were performed during house to house visit of the field study. Statistical analysis included nonparametric (two-tailed) Mann-Whitney U and Spearman rank test, as appropriate (significance: p<0.05).
Results: Serum GM-CSF [69.64 (46.67, 97.48); 36.78 (30.07, 53.88), p=0.014], IFN- γ [51.06 (17.00, 84.86); 11.70 (3.18, 32.81), p=0.017], IL-4 [9.09 (1.8, 19.9); 1.8 (1.8, 4.46); p=0.024], and TNF- α [20.68 (5.5, 29.26); 3.5 (3.5, 4.5); p<0.001] concentrations (pg/ml) were increased in TS COPD subjects compared to TS CONTROL. A weak correlation between lung function parameters and cytokine concentrations was detected.
Conclusion: Our pilot study reveals GM-CSF, IFN- γ , IL-4, and TNF- α as plausible COPD susceptibility biomarkers within the investigated South Indian population that needs to be validated in a larger cohort.
Databáze: MEDLINE
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