Megahertz data collection from protein microcrystals at an X-ray free-electron laser.

Autor:	Grünbein ML; Max Planck Institute for Medical Research, Jahnstrasse 29, 69120, Heidelberg, Germany., Bielecki J; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Gorel A; Max Planck Institute for Medical Research, Jahnstrasse 29, 69120, Heidelberg, Germany., Stricker M; Max Planck Institute for Medical Research, Jahnstrasse 29, 69120, Heidelberg, Germany., Bean R; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Cammarata M; Department of Physics, UMR 625, UBL, University of Rennes 1, 35042, Rennes, France., Dörner K; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Fröhlich L; Deutsches Elektronensynchrotron DESY, Notkestraße 85, 22607, Hamburg, Germany., Hartmann E; Max Planck Institute for Medical Research, Jahnstrasse 29, 69120, Heidelberg, Germany., Hauf S; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Hilpert M; Max Planck Institute for Medical Research, Jahnstrasse 29, 69120, Heidelberg, Germany., Kim Y; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Kloos M; Max Planck Institute for Medical Research, Jahnstrasse 29, 69120, Heidelberg, Germany., Letrun R; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Messerschmidt M; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.; BioXFEL STC, 700 Ellicott Street, Buffalo, NY, 14203, USA., Mills G; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.; ARC Centre of Excellence for Advanced Molecular Imaging, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia., Nass Kovacs G; Max Planck Institute for Medical Research, Jahnstrasse 29, 69120, Heidelberg, Germany., Ramilli M; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Roome CM; Max Planck Institute for Medical Research, Jahnstrasse 29, 69120, Heidelberg, Germany., Sato T; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.; Center for Free-Electron Laser Science, Deutsches Elektronensynchrotron, Notkestraße 85, 22607, Hamburg, Germany., Scholz M; Deutsches Elektronensynchrotron DESY, Notkestraße 85, 22607, Hamburg, Germany., Sliwa M; Laboratoire de Spectrochimie Infrarouge et Raman, CNRS, UMR 8516, Université de Lille, 59000, Lille, France., Sztuk-Dambietz J; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Weik M; Institut de Biologie Structurale, Université Grenoble Alpes, CEA, CNRS, 38044, Grenoble, France., Weinhausen B; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Al-Qudami N; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Boukhelef D; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Brockhauser S; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.; Biological Research Centre (BRC), Hungarian Academy of Sciences, Temesvári krt. 62, Szeged, 6726, Hungary., Ehsan W; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Emons M; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Esenov S; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Fangohr H; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Kaukher A; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Kluyver T; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Lederer M; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Maia L; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Manetti M; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Michelat T; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Münnich A; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Pallas F; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Palmer G; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Previtali G; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Raab N; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Silenzi A; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Szuba J; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Venkatesan S; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Wrona K; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Zhu J; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Doak RB; Max Planck Institute for Medical Research, Jahnstrasse 29, 69120, Heidelberg, Germany., Shoeman RL; Max Planck Institute for Medical Research, Jahnstrasse 29, 69120, Heidelberg, Germany., Foucar L; Max Planck Institute for Medical Research, Jahnstrasse 29, 69120, Heidelberg, Germany., Colletier JP; Institut de Biologie Structurale, Université Grenoble Alpes, CEA, CNRS, 38044, Grenoble, France., Mancuso AP; European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany., Barends TRM; Max Planck Institute for Medical Research, Jahnstrasse 29, 69120, Heidelberg, Germany. Thomas.Barends@mpimf-heidelberg.mpg.de., Stan CA; Department of Physics, Rutgers University Newark, 101 Warren Street, Newark, NJ, 07102, USA. claudiu.stan@rutgers.edu., Schlichting I; Max Planck Institute for Medical Research, Jahnstrasse 29, 69120, Heidelberg, Germany. Ilme.Schlichting@mpimf-heidelberg.mpg.de.
Jazyk:	angličtina
Zdroj:	Nature communications [Nat Commun] 2018 Aug 28; Vol. 9 (1), pp. 3487. Date of Electronic Publication: 2018 Aug 28.
DOI:	10.1038/s41467-018-05953-4
Abstrakt:	X-ray free-electron lasers (XFELs) enable novel experiments because of their high peak brilliance and femtosecond pulse duration. However, non-superconducting XFELs offer repetition rates of only 10-120 Hz, placing significant demands on beam time and sample consumption. We describe serial femtosecond crystallography experiments performed at the European XFEL, the first MHz repetition rate XFEL, delivering 1.128 MHz X-ray pulse trains at 10 Hz. Given the short spacing between pulses, damage caused by shock waves launched by one XFEL pulse on sample probed by subsequent pulses is a concern. To investigate this issue, we collected data from lysozyme microcrystals, exposed to a ~15 μm XFEL beam. Under these conditions, data quality is independent of whether the first or subsequent pulses of the train were used for data collection. We also analyzed a mixture of microcrystals of jack bean proteins, from which the structure of native, magnesium-containing concanavalin A was determined.
Databáze:	MEDLINE
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