| Autor: |
Suong NT; Institute of Marine Science, University of Auckland, Auckland 1142, New Zealand., Banks JC, Webb SC, Jeffs A, Wakeman KC, Fidler A |
| Jazyk: |
angličtina |
| Zdroj: |
Diseases of aquatic organisms [Dis Aquat Organ] 2018 Aug 14; Vol. 129 (3), pp. 199-205. |
| DOI: |
10.3354/dao03244 |
| Abstrakt: |
Described here is a polymerase chain reaction (PCR) test to detect the apicomplexan-X (APX) parasite of a flat oyster species, Ostrea chilensis, endemic to New Zealand. The test primers target sequences in the in situ hybridisation probes identified to bind specifically to APX 18S rRNA and amplify a 723 bp DNA product. The test did not amplify 18S rRNA gene sequences of other apicomplexan species, including Toxoplasma gondii, Neospora caninum, Selenidium spp., Cephaloidophorida spp., Lecudina spp. and Thiriotia sp. Of 73 flat oysters identified by histology to be infected with APX at different severities, 69 (95%) tested PCR-positive. Failure to amplify an internal control indicated the presence of PCR inhibitors in the 4 PCR-negative samples. The high analytical sensitivity, specificity and speed of the PCR test should make it a useful tool for detecting APX. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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