In Vivo genotoxicity of a commercial C.I. Disperse Red 1 dye.

Autor: Fernandes FH; Department of Pathology, Medical School, São Paulo State University, Botucatu, São Paulo, Brazil.; National Institute for Alternative Technologies of Detection, Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM), Institute of Chemistry Araraquara, Araraquara, São Paulo, Brazil., Botasso-Nasciutti MO; Department of Pathology, Medical School, São Paulo State University, Botucatu, São Paulo, Brazil., Svio ALV; Department of Pathology, Medical School, São Paulo State University, Botucatu, São Paulo, Brazil., Souza LDCM; Department of Pathology, Medical School, São Paulo State University, Botucatu, São Paulo, Brazil., Fernandes-Cal JR; Department of Pathology, Medical School, São Paulo State University, Botucatu, São Paulo, Brazil., Cardoso FF; Department of Physics and Biophysics, Bioscience Institute, São Paulo State University, Botucatu, São Paulo, Brazil., Fontes MRM; Department of Physics and Biophysics, Bioscience Institute, São Paulo State University, Botucatu, São Paulo, Brazil., Albuquerque AF; School of Technology, State University of Campinas, Limeira, São Paulo, Brazil., Munari CC; Department of Pathology, Medical School, São Paulo State University, Botucatu, São Paulo, Brazil., Kummrow F; Institute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo, Diadema, São Paulo, Brazil., Umbuzeiro GA; School of Technology, State University of Campinas, Limeira, São Paulo, Brazil., Salvadori DMF; Department of Pathology, Medical School, São Paulo State University, Botucatu, São Paulo, Brazil.; National Institute for Alternative Technologies of Detection, Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM), Institute of Chemistry Araraquara, Araraquara, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Environmental and molecular mutagenesis [Environ Mol Mutagen] 2018 Dec; Vol. 59 (9), pp. 822-828. Date of Electronic Publication: 2018 Aug 27.
DOI: 10.1002/em.22226
Abstrakt: Color Index (C.I.) Disperse Red 1 (DR1) is a widely used textile azo dye found in rivers. As it may not be completely removed by conventional treatments, humans can be exposed through drinking water. Studies have supported in vitro toxicity and mutagenicity of commercial DR1. This study aimed to investigate the mutagenic and toxicogenomic effects of commercial DR1 in multiple tissues/organs of Swiss male mice. For that, animals were orally exposed to the dye (by gavage), at single doses of 0.0005, 0.005, 0.5, 50, or 500 mg/kg bw. The two lowest doses were equivalent to the ones found in two Brazilian rivers receiving influx of textile discharges. Cytotoxicity, micronucleated cell frequencies (for all doses tested), primary DNA damage (comet assay), and gene expression profiling of (0.0005 and 0.005 mg/kg of bw) were investigated 24 hr after animal exposure to commercial DR1. Data showed increased frequencies of micronucleated polychromatic erythrocytes in bone marrow cells after treatment with 0.5 and 50 mg/kg bw. At 0.005 mg/kg bw, commercial DR1 induced an increase of primary DNA damage in liver, but not in kidney cells. Additionally, upregulation of genes involved in the inflammatory process (IL1B) (0.0005 and 0.005 mg/kg bw) and cell-cycle control (CDKN1A) in liver cells, and apoptosis (BCL2 and BAX) in leukocytes (0.005 mg/kg bw) were also detected. In conclusion, the commercial DR1 was genotoxic (chromosome aberrations and primary DNA damage) and modulated gene expression in mice, and such effects were dependent on the doses and tissues analyzed. Environ. Mol. Mutagen. 59:822-828, 2018. © 2018 Wiley Periodicals, Inc.
(© 2018 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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