Analysis of Polymorphisms in Genes Differentially Expressed in the Enamel of Mice with Different Genetic Susceptibilities to Dental Fluorosis.

Autor: Charone S; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil., Küchler EC; Department of Pediatric Dentistry, Ribeirão Preto Dental School, University of São Paulo, Ribeirão Preto, Brazil., Leite AL; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil., Silva Fernandes M; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil., Taioqui Pelá V; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil., Martini T; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil., Brondino BM; Department of Prosthodontics, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil., Magalhães AC; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil., Dionisio TJ; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil., F Santos C; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil., Buzalaf MAR; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazilmbuzalaf@fob.usp.br.
Jazyk: angličtina
Zdroj: Caries research [Caries Res] 2019; Vol. 53 (2), pp. 228-233. Date of Electronic Publication: 2018 Aug 27.
DOI: 10.1159/000491554
Abstrakt: Genes expressed during amelogenesis are candidates to increase the risk of dental fluorosis (DF). Thus, this study aimed to evaluate the association between polymorphisms in enamel development genes and susceptibility to DF in mice. Mice of both sexes, representing strains 129P3/J (n = 20; resistant to DF) and A/J (n = 20; susceptible to DF), were divided into 2 groups. Each strain received a diet with a low concentration of fluoride (F) and drinking water containing 0 or 50 mg/L of F for 6 weeks. Clinical evaluation and analysis of Vickers enamel microhardness of the incisors were performed. Livers were collected for genomic DNA extraction. Seventeen genetic polymorphisms in Amelx, Ambn, Ambn, Col14a1, Col1a1, Col5a2, Enam, Fam20a, Fam83h, Foxo1, Klk4, Mmp20, Serpinf1, Serpinh1, Smad3, Tuft1, and Wdr72 were genotyped by real-time PCR using Taqman chemistry. Overrepresentation of alleles and genotypes in DF was evaluated using the χ2 test with an alpha of 5%. The clinical aspects of the enamel and the surface enamel microhardness confirmed the DF condition. In the polymorphisms rs29569969, rs13482592, and rs13480057 in Ambn, Col14a1, and Mmp20, respectively, genotype and allele distributions were statistically significantly different between A/J and 129P3/J strains (p < 0.05). In conclusion, polymorphisms in Ambn, Col14a1, and Mmp20 are associated with the susceptibility to DF.
(© 2018 S. Karger AG, Basel.)
Databáze: MEDLINE
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