Hypoxic preconditioning effect on stromal cells derived factor-1 and C-X-C chemokine receptor type 4 expression in Wistar rat's ( Rattus norvegicus ) bone marrow mesenchymal stem cells ( in vitro study).

Autor: Mulyani SWM; Department of Dentomaxillofacial Radiology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia., Ernawati DS; Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia., Astuti ER; Department of Dentomaxillofacial Radiology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia., Rantam FA; Stem Cell Research Center and Development, Airlangga University Surabaya, Indonesia.; Lab of Virology and Immunology, Department of Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Jazyk: angličtina
Zdroj: Veterinary world [Vet World] 2018 Jul; Vol. 11 (7), pp. 965-970. Date of Electronic Publication: 2018 Jul 19.
DOI: 10.14202/vetworld.2018.965-970
Abstrakt: Aim: To examine the effect of hypoxic preconditions on the ability of bone marrow stem cells culture mediated expression C-X-C chemokine receptor type 4 (CXCR4) and stromal cells derived factor-1 (SDF-1) in vitro .
Materials and Methods: Bone marrow mesenchymal stem cells (BMSCs) were derived from 12 femurs of 200 g Wistar male rats. The animals were euthanized before BMSCs isolation. BMSCs were divided into two groups, control group: Normoxic condition 21% O 2 and treatment group: Hypoxic condition 1% O 2 . The characterization of BMSCs was analyzed using flow cytometry by cluster differentiation 34 and cluster differentiation 105. The expression of CXCR4 and SDF-1 measured using immunocytochemistry immunofluorescence label after 48-h incubation in a low-tension oxygen chamber with an internal atmosphere consisting of 95% N 2, 5% CO 2 , and 1% O 2 . All data were subjected to a normality test and then analyzed using t-test statistic (p<0.05).
Results: The characterization of bone marrow stem cells showed positive cluster differentiation 34 and cluster differentiation 105. A hypoxic precondition (1% O 2 ) in culture increases CXCR4 (p=0.000) and SDF-1 expression than normoxic conditions (p=0.000) (p<0.05).
Conclusion: Hypoxic preconditioning with 1% O 2 increase CXCR4 and SDF1 expression.
Databáze: MEDLINE
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