| Autor: |
Dubinina EE, Sal'nikova LA, Efimova LF, Evarestov PA |
| Jazyk: |
ruština |
| Zdroj: |
Ukrainskii biokhimicheskii zhurnal (1978) [Ukr Biokhim Zh (1978)] 1986 May-Jun; Vol. 58 (3), pp. 31-6. |
| Abstrakt: |
The superoxide-dismutase (EC 1.15.1.1) activity was revealed in blood plasma after its successive treatment with alcohol, chloroform and one- or two-substituted phosphate. Its value is the highest when treating plasma with KH2PO4. The boiling of the supernatant liquid obtained after addition of K2HPO4 leads to a complete loss of the activity. The treatment of plasma with KH2PO4 provides higher stability of the enzyme to thermal actions. The analysis of EPR-spectra has shown the presence of different complex compounds of copper ions in the supernatant liquid. Certain experiments with the use of aqueous solutions of CuCl2 and histidine have shown that Cu2+ inhibits the superoxide-dismutase activity in samples treated with KH2PO4 and increases it in samples treated with K2HPO4. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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