Generation of two iPSC lines (IMGTi001-A and IMGTi001-B) from human skin fibroblasts with ring chromosome 22.

Autor:	Nikitina TV; Research Institute of Medical Genetics, Tomsk NRMC, Tomsk, Russia. Electronic address: t.nikitina@medgenetics.ru., Menzorov AG; Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, Russia., Kashevarova AA; Research Institute of Medical Genetics, Tomsk NRMC, Tomsk, Russia., Gridina MM; Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia., Khabarova AA; Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia., Yakovleva YS; Research Institute of Medical Genetics, Tomsk NRMC, Tomsk, Russia., Lopatkina ME; Research Institute of Medical Genetics, Tomsk NRMC, Tomsk, Russia., Kizilova EA; Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia., Vasilyev SA; Research Institute of Medical Genetics, Tomsk NRMC, Tomsk, Russia., Serov OL; Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, Russia., Lebedev IN; Research Institute of Medical Genetics, Tomsk NRMC, Tomsk, Russia.
Jazyk:	angličtina
Zdroj:	Stem cell research [Stem Cell Res] 2018 Aug; Vol. 31, pp. 244-248. Date of Electronic Publication: 2018 Aug 16.
DOI:	10.1016/j.scr.2018.08.012
Abstrakt:	Skin fibroblasts from a patient with intellectual disability and ring chromosome 22 were reprogrammed into induced pluripotent stem cells (iPSCs) to establish a clonal stem cell lines, IMGTi001-A (iTAF5-29) and IMGTi001-B (iTAF5-32). Because of ring chromosome mitotic instability these cell lines show mosaic karyotypes with 46,XX,r(22) in >83% cells, 45,XX,-22 as minor class and sporadically cells with other karyotypes. Differentiation in derivatives of all three germ layers was shown in teratoma assay for IMGTi001-A, and in embryoid bodies for both cell lines. To our knowledge, human iPSC lines with ring chromosome are described for the first time. (Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze:	MEDLINE
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