Structure of acid-extractable polysaccharides of tree greenery of Picea abies.

Autor: Shakhmatov EG; Institute of Chemistry, Komi Science Centre, Ural Branch of the Russian Academy of Sciences, Pervomaiskaya st. 48, Syktyvkar 167982, Russia., Belyy VA; Institute of Chemistry, Komi Science Centre, Ural Branch of the Russian Academy of Sciences, Pervomaiskaya st. 48, Syktyvkar 167982, Russia., Makarova EN; Institute of Chemistry, Komi Science Centre, Ural Branch of the Russian Academy of Sciences, Pervomaiskaya st. 48, Syktyvkar 167982, Russia. Electronic address: makarowa.elena-ma@yandex.ru.
Jazyk: angličtina
Zdroj: Carbohydrate polymers [Carbohydr Polym] 2018 Nov 01; Vol. 199, pp. 320-330. Date of Electronic Publication: 2018 Jul 10.
DOI: 10.1016/j.carbpol.2018.07.027
Abstrakt: The polysaccharide PA A was isolated from tree greenery of spruce Picea abies by the extraction with HCl solution. Monosaccharide analysis PA A revealed high content of uronic acid residues (45%), arabinose (19.5%) and galactose (8.6%). It was shown that this polysaccharide contained polymers of various structures. The major component of which were pectic polysaccharides represented mainly by partly methyl-esterified and partly acetylated 1,4-α-D-galacturonan and by minor parts of partly 2-O- and/or 3-O- acetylated rhamnogalacturonan-I (RG-I). The side carbohydrate chains of the branched region of RG-I were represented predominantly by highly branched 1,5-α-L-arabinan and minor portions of 1,4-β-D-galactan. In addition to the dominant pectins, the isolated polysaccharide also contained arabinogalactan proteins (AGP) and binding glycans of the glucuronoarabinoxylan (GAX), glucomannan (GM) and xyloglucan classes, which indicated a close interaction of these polysaccharides in the cell walls. It can be concluded that, at least a part of the pectin is strongly associated with the AGP and binding glycans of the GAX and GM classes. However, neither GM nor GAX were associated with RG-I.
(Copyright © 2018 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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