Impact of Clinical Use of Parenteral Lipid Emulsions on Bile Acid Metabolism and Composition in Neonatal Piglets.

Autor: Lavallee CM; Department of Pediatrics, University of Alberta, Edmonton, Alberta, Canada.; Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada., Lim DW; Department of Pediatrics, University of Alberta, Edmonton, Alberta, Canada., Wizzard PR; Department of Pediatrics, University of Alberta, Edmonton, Alberta, Canada., Mazurak VC; Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada., Mi S; Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada.; Institute of Food Science and Technology, Chinese Academy of Agricultural Science, Beijing, China., Curtis JM; Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada., Willing BP; Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada., Yap JY; Department of Pediatrics, University of Alberta, Edmonton, Alberta, Canada., Wales PW; Department of Pediatrics, University of Alberta, Edmonton, Alberta, Canada.; Department of Surgery, University of Toronto, Toronto, Ontario, Canada., Turner JM; Department of Pediatrics, University of Alberta, Edmonton, Alberta, Canada.
Jazyk: angličtina
Zdroj: JPEN. Journal of parenteral and enteral nutrition [JPEN J Parenter Enteral Nutr] 2019 Jul; Vol. 43 (5), pp. 668-676. Date of Electronic Publication: 2018 Aug 23.
DOI: 10.1002/jpen.1437
Abstrakt: Background: Neonates with intestinal failure dependent on parenteral nutrition (PN) are at risk of intestinal failure-associated liver disease (IFALD). PN lipid composition relates to the risk of IFALD, but the mechanisms are poorly understood. We investigated the effects of soybean oil (SO), a mixed-lipid (ML) emulsion containing fish oil (FO), and a pure FO. We hypothesized FO-containing PN lipids would result in increased gene expression of canalicular bile acid transporters and a larger, more hydrophilic bile acid pool, predictive of increased bile flow.
Methods: Neonatal piglets were allocated to receive 1 of SO, ML, or FO throughout 14 days of PN feeding. Relative expression of genes involved in bile acid synthesis and transport were determined through quantitative polymerase chain reaction. Bile secreted from the liver was collected and measured. Bile acid composition was determined using tandem mass spectrometry. Regression analysis was used to determine predictors of bile flow.
Results: PN reduced bile acid secretion (P < .001). FO-containing PN lipids were associated with greater expression of bile acid and organic solute transport genes (P < .05) and greater secretion of hydrophobic bile acids (P < .001). Farnesoid X receptor (P = .01), bile salt export pump (P < .01), multidrug resistant protein 2 (P < .01), and unconjugated hyocholic acid (P < .001) independently predicted bile flow.
Conclusions: PN lipid modulation altered bile acid metabolism and composition. These alterations may explain the hepatoprotective effects of FO-containing PN lipids and support their use in the prevention and treatment of IFALD.
(© 2018 American Society for Parenteral and Enteral Nutrition.)
Databáze: MEDLINE
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