Piezo1 forms a slowly-inactivating mechanosensory channel in mouse embryonic stem cells.

Autor:	Del Mármol JI; Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, Howard Hughes Medical Institute, New York, United States., Touhara KK; Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, Howard Hughes Medical Institute, New York, United States., Croft G; Laboratory of Stem Cell Biology and Molecular Embryology, Rockefeller University, New York, United States., MacKinnon R; Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, Howard Hughes Medical Institute, New York, United States.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2018 Aug 22; Vol. 7. Date of Electronic Publication: 2018 Aug 22.
DOI:	10.7554/eLife.33149
Abstrakt:	Piezo1 is a mechanosensitive (MS) ion channel with characteristic fast-inactivation kinetics. We found a slowly-inactivating MS current in mouse embryonic stem (mES) cells and characterized it throughout their differentiation into motor-neurons to investigate its components. MS currents were large and slowly-inactivating in the stem-cell stage, and became smaller and faster-inactivating throughout the differentiation. We found that Piezo1 is expressed in mES cells, and its knockout abolishes MS currents, indicating that the slowly-inactivating current in mES cells is carried by Piezo1. To further investigate its slow inactivation in these cells, we cloned Piezo1 cDNA from mES cells and found that it displays fast-inactivation kinetics in heterologous expression, indicating that sources of modulation other than the aminoacid sequence determine its slow kinetics in mES cells. Finally, we report that Piezo1 knockout ES cells showed a reduced rate of proliferation but no significant differences in other markers of pluripotency and differentiation. Competing Interests: Jd, KT, GC, RM No competing interests declared (© 2018, del Mármol et al.)
Databáze:	MEDLINE
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