A low-cost paper-based synthetic biology platform for analyzing gut microbiota and host biomarkers.

Autor: Takahashi MK; Institute for Medical Engineering and Science, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA, 02139, USA., Tan X; Institute for Medical Engineering and Science, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA, 02139, USA.; Division of Gastroenterology, Massachusetts General Hospital, 55 Fruit Street, Boston, MA, 02114, USA.; Harvard Medical School, 25 Shattuck St, Boston, MA, 02115, USA.; Wyss Institute for Biologically Inspired Engineering, Harvard University, 3 Blackfan Circle, Boston, MA, 02115, USA.; Broad Institute of MIT and Harvard, 415 Main St, Cambridge, MA, 02142, USA., Dy AJ; Institute for Medical Engineering and Science, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA, 02139, USA.; Broad Institute of MIT and Harvard, 415 Main St, Cambridge, MA, 02142, USA.; Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA, 02139, USA., Braff D; Institute for Medical Engineering and Science, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA, 02139, USA.; Department of Biomedical Engineering, Boston University, 44 Cummington Mall, Boston, MA, 02215, USA., Akana RT; Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA, 02139, USA., Furuta Y; Institute for Medical Engineering and Science, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA, 02139, USA.; Division of Infection and Immunity, Research Center for Zoonosis Control, Hokkaido University, North 20, West 10 Kita-ku, Sapporo, 001-0020, Japan., Donghia N; Wyss Institute for Biologically Inspired Engineering, Harvard University, 3 Blackfan Circle, Boston, MA, 02115, USA., Ananthakrishnan A; Division of Gastroenterology, Massachusetts General Hospital, 55 Fruit Street, Boston, MA, 02114, USA.; Harvard Medical School, 25 Shattuck St, Boston, MA, 02115, USA., Collins JJ; Institute for Medical Engineering and Science, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA, 02139, USA. jimjc@mit.edu.; Wyss Institute for Biologically Inspired Engineering, Harvard University, 3 Blackfan Circle, Boston, MA, 02115, USA. jimjc@mit.edu.; Broad Institute of MIT and Harvard, 415 Main St, Cambridge, MA, 02142, USA. jimjc@mit.edu.; Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA, 02139, USA. jimjc@mit.edu.; Synthetic Biology Center, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA, 02139, USA. jimjc@mit.edu.; Harvard-MIT Program in Health Sciences and Technology, 77 Massachusetts Ave, Cambridge, MA, 02139, USA. jimjc@mit.edu.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2018 Aug 21; Vol. 9 (1), pp. 3347. Date of Electronic Publication: 2018 Aug 21.
DOI: 10.1038/s41467-018-05864-4
Abstrakt: There is a need for large-scale, longitudinal studies to determine the mechanisms by which the gut microbiome and its interactions with the host affect human health and disease. Current methods for profiling the microbiome typically utilize next-generation sequencing applications that are expensive, slow, and complex. Here, we present a synthetic biology platform for affordable, on-demand, and simple analysis of microbiome samples using RNA toehold switch sensors in paper-based, cell-free reactions. We demonstrate species-specific detection of mRNAs from 10 different bacteria that affect human health and four clinically relevant host biomarkers. We develop a method to quantify mRNA using our toehold sensors and validate our platform on clinical stool samples by comparison to RT-qPCR. We further highlight the potential clinical utility of the platform by showing that it can be used to rapidly and inexpensively detect toxin mRNA in the diagnosis of Clostridium difficile infections.
Databáze: MEDLINE