Evaluation of sperm head dimensions and chromatin integrity of epididymal sperm from domestic cats using the toluidine blue technique.

Autor: Alves IP; Department of Medicine, Surgery and Animal Reproduction, School of Veterinary Medicine, São Paulo State University (UNESP), Araçatuba, São Paulo, Brazil. Electronic address: izapazzoto@hotmail.com., Cancelli CHB; Department of Medicine, Surgery and Animal Reproduction, School of Veterinary Medicine, São Paulo State University (UNESP), Araçatuba, São Paulo, Brazil. Electronic address: caecancelli@hotmail.com., Grassi TLM; Department of Support, Production and Animal Health, School of Veterinary Medicine, São Paulo State University (UNESP), Araçatuba, São Paulo, Brazil. Electronic address: thiagograssi@hotmail.com., Oliveira PRH; Department of Medicine, Surgery and Animal Reproduction, School of Veterinary Medicine, São Paulo State University (UNESP), Araçatuba, São Paulo, Brazil. Electronic address: patihegge@hotmail.com., Franciscato DA; University of Araraquara - UNIARA - School of Veterinary Medicine, Araraquara, São Paulo, Brazil. Electronic address: douglas_aug@yahoo.com.br., Carreira JT; Department of Agricultural Sciences, Federal Institute of Minas Gerais, Bambuí, Minas Gerais, Brazil. Electronic address: janaina.carreira@ifmg.edu.br., Koivisto MB; Department of Medicine, Surgery and Animal Reproduction, School of Veterinary Medicine, São Paulo State University (UNESP), Araçatuba, São Paulo, Brazil. Electronic address: marion.koivisto@unesp.br.
Jazyk: angličtina
Zdroj: Animal reproduction science [Anim Reprod Sci] 2018 Oct; Vol. 197, pp. 33-39. Date of Electronic Publication: 2018 Aug 06.
DOI: 10.1016/j.anireprosci.2018.08.001
Abstrakt: When using assisted reproductive technologies, there is seldom an evaluation of DNA integrity during sperm analysis, which is an important variable for proper embryo development. The toluidine blue staining technique allows the simultaneous evaluation of sperm chromatin and sperm head dimensions. The objectives of this study were to evaluate the applicability of the toluidine blue staining method for analyzing DNA abnormalities in epididymal sperm (from the caput, corpus, and cauda) of cats and to investigate the correlations among DNA condensation, morphology, and sperm head dimensions. The DNA alteration indexes were obtained using the toluidine blue and acridine orange techniques, and comparisons of these indexes indicated there was a 65.4% (r = 0.654; P < 0.001) correlation. The sperm from the cauda had greater chromatin stability (97.9%) than the sperm from the epididymal head (92.1%; P = 0.0023). There, however, was no difference in chromatin stability between sperm obtained from the corpus and cauda regions, indicating that these sperm were already mature. The sperm head dimension was correlated with chromatin decondensation, and the sperm head size decreased as the sperm were transported through the three epididymal regions (P < 0.0001). In addition, the percentage of sperm that were deficient in chromatin condensation decreased as the sperm were transported through the epididymal caput, corpus and cauda (26.4, 15.7, and 3.4%, respectively; P < 0.0001). Thus, the sperm head size predicts the quality of chromatin condensation in sperm cells.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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