Molecular epidemiology of isolates with multiple mcr plasmids from a pig farm in Great Britain: the effects of colistin withdrawal in the short and long term.

Autor: Duggett NA; Animal and Plant Health Agency, Weybridge, New Haw, Addlestone, Surrey, UK., Randall LP; Animal and Plant Health Agency, Weybridge, New Haw, Addlestone, Surrey, UK., Horton RA; Animal and Plant Health Agency, Weybridge, New Haw, Addlestone, Surrey, UK., Lemma F; Animal and Plant Health Agency, Weybridge, New Haw, Addlestone, Surrey, UK., Kirchner M; Animal and Plant Health Agency, Weybridge, New Haw, Addlestone, Surrey, UK., Nunez-Garcia J; Animal and Plant Health Agency, Weybridge, New Haw, Addlestone, Surrey, UK., Brena C; Animal and Plant Health Agency (Thirsk), West House, Station Road, Thirsk, North Yorkshire, UK., Williamson SM; Animal and Plant Health Agency (Bury St Edmunds), Rougham Hill, Bury St Edmunds, Suffolk, UK., Teale C; Animal and Plant Health Agency (Shrewsbury), Kendal Road, Shrewsbury, UK., Anjum MF; Animal and Plant Health Agency, Weybridge, New Haw, Addlestone, Surrey, UK.
Jazyk: angličtina
Zdroj: The Journal of antimicrobial chemotherapy [J Antimicrob Chemother] 2018 Nov 01; Vol. 73 (11), pp. 3025-3033.
DOI: 10.1093/jac/dky292
Abstrakt: Background: The environment, including farms, might act as a reservoir for mobile colistin resistance (mcr) genes, which has led to calls for reduction of usage in livestock of colistin, an antibiotic of last resort for humans.
Objectives: To establish the molecular epidemiology of mcr Enterobacteriaceae from faeces of two cohorts of pigs, where one group had initially been treated with colistin and the other not, over a 5 month period following stoppage of colistin usage on a farm in Great Britain; faecal samples were also taken at ∼20 months.
Methods: mcr-1 Enterobacteriaceae were isolated from positive faeces and was WGS performed; conjugation was performed on selected Escherichia coli and colistin MICs were determined.
Results: E. coli of diverse ST harbouring mcr-1 and multiple resistance genes were isolated over 5 months from both cohorts. Two STs, from treated cohorts, contained both mcr-1 and mcr-3 plasmids, with some isolates also harbouring multiple copies of mcr-1 on different plasmids. The mcr-1 plasmids grouped into four Inc types (X4, pO111, I2 and HI2), with mcr-3 found in IncP. Multiple copies of mcr plasmids did not have a noticeable effect on colistin MIC, but they could be transferred simultaneously to a Salmonella host in vitro. Neither mcr-1 nor mcr-3 was detected in samples collected ∼20 months after colistin cessation.
Conclusions: We report for the first known time on the presence in Great Britain of mcr-3 from MDR Enterobacteriaceae, which might concurrently harbour multiple copies of mcr-1 on different plasmids. However, control measures, including stoppage of colistin, can successfully mitigate long-term on-farm persistence.
Databáze: MEDLINE