[Effects of serine/threonine-protein kinase B-Raf-activated long-chain non-coding RNA on apoptosis and autophagy in thyroid carcinoma cells].

Autor:	Liu T; Department of Anatomy, Department of Basic Medicine, Shangqiu Medical College, Shangqiu Henan 476100, China., Zhao Y; Department of Anatomy, Department of Basic Medicine, Shangqiu Medical College, Shangqiu Henan 476100, China., Wang L; Department of Anatomy, Department of Basic Medicine, Shangqiu Medical College, Shangqiu Henan 476100, China., Jia H; Department of Endocrinology, Zhengzhou University People's Hospital, Zhengzhou 450003, China., Cui D; Department of Anatomy, Department of Basic Medicine, Shangqiu Medical College, Shangqiu Henan 476100, China., Si Y; Department of Anatomy, Department of Basic Medicine, Shangqiu Medical College, Shangqiu Henan 476100, China., Wang H; Department of Oncology, First Affiliated Hospital of Shangqiu Medical College, Shangqiu Henan 476100, China., Xue H; Department of General Surgery, First Affiliated Hospital of Xinxiang Medical College, Xinxiang Henan 453100, China.
Jazyk:	čínština
Zdroj:	Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences [Zhong Nan Da Xue Xue Bao Yi Xue Ban] 2018 Jul 28; Vol. 43 (7), pp. 747-753.
DOI:	10.11817/j.issn.1672-7347.2018.07.008
Abstrakt:	Objective: To investigate the effect of serine/threonine-protein kinase B-Raf (BRAF)-activated long-chain non-coding RNA (lncRNA-BANCR) on apoptosis and autophagy in thyroid carcinoma cells and the underlying mechanisms. Methods: RT-PCR was used to detect the expression of lncRNA-BANCR in thyroid carcinoma and normal thyroid tissues. The association between lncRNA-BANCR and clinicopathological data was analyzed in patients with thyroid cancer. Cell counting kit-8 (CCK-8) was used to detect the effect of lncRNA-BANCR on the proliferation of thyroid cancer cells. The effect of lncRNA-BANCR on the apoptosis of thyroid carcinoma cells was detected by flow cytometry. Transwell invasion assay was used to detect the effect of lncRNA-BANCR on the invasive ability of thyroid cancer cells. Western blot was used to detect the changes of autophagy proteins LC3-I and LC3-II after the lncRNA-BANCR expression was suppressed. Results: Compared with normal thyroid tissues, the expression level of lncRNA-BANCR in thyroid carcinoma tissues was elevated (P<0.05). The expression of lncRNA-BANCR was positively related to the pathological stage of thyroid carcinoma and the lymph node metastasis. Inhibition of lncRNA-BANCR expression attenuated the proliferation and invasion ability of thyroid cancer cells (both P<0.05); but the apoptosis was enhanced (P<0.05); the expression levels of autophagy protein LC3-I and LC3-II were also increased (P<0.05). Conclusion: The expression level of lncRNA-BANCR affects the proliferation, invasion and apoptosis of thyroid cancer cells through modulation of autophagy behavior.
Databáze:	MEDLINE
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