Calliandra surinamensis lectin (CasuL) does not impair the functionality of mice splenocytes, promoting cell signaling and cytokine production.

Autor: Procópio TF; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420, Recife, Brazil., de Siqueira Patriota LL; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420, Recife, Brazil., da Silva Barros BR; Departamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420, Recife, Brazil., de Souza Aguiar LM; Departamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420, Recife, Brazil., de Lorena VMB; Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, Pernambuco, Brazil., Paiva PMG; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420, Recife, Brazil., de Melo CML; Departamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420, Recife, Brazil., Napoleão TH; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420, Recife, Brazil. Electronic address: thiagohn86@yahoo.com.br.
Jazyk: angličtina
Zdroj: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2018 Nov; Vol. 107, pp. 650-655. Date of Electronic Publication: 2018 Aug 15.
DOI: 10.1016/j.biopha.2018.08.043
Abstrakt: CasuL is a lectin (carbohydrate-binding protein) isolated from the leaf pinnulae of Calliandra surinamensis that is toxic against cancer cells. In this study, the effects of CasuL on the activation of immune cells were evaluated in BALB/c mice splenocytes. Assays measuring the changes in cytosolic calcium concentration ([Ca 2+ ] cyt ), mitochondrial membrane potential (ΔΨm), and reactive oxygen species (ROS) levels associated with cell viability, proliferation, and cytokine and nitric oxide production were performed. The lectin (3.12-100 μg/mL) did not induce apoptosis or necrosis of splenocytes, and treatment for 48 h at 12.5 μg/mL stimulated cell proliferation. High cytosolic ROS levels were found in cells incubated with CasuL (12.5 μg/mL), but it did not affect [Ca 2+ ] cyt , mitochondrial ROS, and ΔΨm levels. Furthermore, CasuL promoted high IL-2 and TNF-α production but did not affect nitric oxide release. In conclusion, CasuL was able to promote oxidative stress in mouse immune cells without inducing cell damage, and stimulated proliferation and cytokine production. These findings suggest the potential use of CasuL in future antitumoral and immunological targets.
(Copyright © 2018 Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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