An ethnobotanical survey and inhibitory effects on NLRP3 inflammasomes/Caspase-1 of herbal recipes' extracts traditionally used in Rwanda for asthma treatment.

Autor: Tomani JCD; University of Rwanda, College of Medicine and Health Sciences, School of Medicine and Pharmacy, Kigali, Rwanda; Université Libre de Bruxelles, Institute for Molecular Biology and Medicine, Laboratory of Embryology and Biotechnology, Gosselies, Belgium., Gainkam LOT; Université Libre de Bruxelles, Institute for Molecular Biology and Medicine, Laboratory of Embryology and Biotechnology, Gosselies, Belgium., Nshutiyayesu S; University of Rwanda, College of Science and Technology, School of Science, Department of Biology, Huye, Rwanda., Mukazayire MJ; National Industrial Research and Development Agency, Pharmaceutical and Chemical Industries Division, Huye, Rwanda., Ribeiro SO; Université Libre de Bruxelles, Faculty of Pharmacy, RD3-Pharmacognosy, Bioanalysis and Drug Discovery Unit, Campus Plaine CP 205/9, 1050 Brussels, Belgium., Stevigny C; Université Libre de Bruxelles, Faculty of Pharmacy, RD3-Pharmacognosy, Bioanalysis and Drug Discovery Unit, Campus Plaine CP 205/9, 1050 Brussels, Belgium., Frederich M; University of Liège, Centre for Interdisciplinary Research on Medicines (CIRM), Laboratory of Pharmacognosy, Liège, Belgium., Muganga R; University of Rwanda, College of Medicine and Health Sciences, School of Medicine and Pharmacy, Kigali, Rwanda., Souopgui J; Université Libre de Bruxelles, Institute for Molecular Biology and Medicine, Laboratory of Embryology and Biotechnology, Gosselies, Belgium. Electronic address: jsouopgu@ulb.ac.be.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2018 Dec 05; Vol. 227, pp. 29-40. Date of Electronic Publication: 2018 Aug 14.
DOI: 10.1016/j.jep.2018.08.016
Abstrakt: Ethnopharmacological Relevance: Respiratory diseases and asthma, in particular, are nowadays a global health problem. In Rwanda, some traditional healers claim to treat asthma with plant-based recipes, though there is no scientific proof so far.
Aim of the Study: Our study aimed at evaluating the toxicity and the anti-inflammatory effect of plant recipes used in Rwanda against asthma in order to select potential candidates for further characterization of the active compounds.
Materials and Methods: Water (aqueous) and methanol-dichloromethane (organic) extracts from selected folkloric recipes were submitted for toxicity test on THP-1 derived macrophages using CellTiter-Glo Luminescent Cell Viability Assay. The evaluation of the anti-inflammatory effect of the plant extracts was carried out using the Caspase-Glo 1 Inflammasome assay on THP-1 -derived macrophages.
Results: Most of both organic and aqueous extract showed more than 95% of cell viability up to 200 µg/ml, except for R03Cn organic extract that inhibited 25% of the cell viability. Plant extracts inhibited caspase-1 activation in THP-1 derived macrophages in a dose-dependent manner. Four extracts (R03Cn and R07Kn aqueous extracts, R10MK and R19Sz organic extracts) strongly downregulated the activation of caspase-1 (more than 70% at 50 µg/ml). In general, organic extracts exhibited better caspase-1 inhibitory effects than their aqueous counterparts.
Conclusions: The inhibition of inflammasome/caspase-1 is one of key mechanisms of action in asthma. Some traditional recipes are active on this mechanism and are thus strong candidates for the treatment of asthma and other inflammasome-mediated diseases. Further investigations are needed to characterize active molecules.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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