The usefulness of short-term high-fat/high salt diet as a model of metabolic syndrome in mice.

Autor: Mendes-Junior LG; Department of Biotechnology, Biotechnology Center, Federal University of Paraiba, João Pessoa, PB, Brazil., Freitas-Lima LC; Institute of Agricultural Sciences, Food Engineering College, Universidade Federal de Minas Gerais (UFMG), Montes Claros, Minas Gerais, Brazil., Oliveira JR; Institute of Agricultural Sciences, Food Engineering College, Universidade Federal de Minas Gerais (UFMG), Montes Claros, Minas Gerais, Brazil., Melo MB; Physiology Department, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil., Feltenberger JD; Medical School, Touro University, Las Vegas, USA., Brandi IV; Institute of Agricultural Sciences, Food Engineering College, Universidade Federal de Minas Gerais (UFMG), Montes Claros, Minas Gerais, Brazil., Carvalho BMA; Institute of Agricultural Sciences, Food Engineering College, Universidade Federal de Minas Gerais (UFMG), Montes Claros, Minas Gerais, Brazil., Guimarães ALS; Lab. Health Science, PPGCS, Universidade Estadual de Montes Claros (Unimontes), Minas Gerais, Brazil., De Paula AMB; Lab. Health Science, PPGCS, Universidade Estadual de Montes Claros (Unimontes), Minas Gerais, Brazil., D'Angelis CEM; Lab. Health Science, PPGCS, Universidade Estadual de Montes Claros (Unimontes), Minas Gerais, Brazil., Campagnole-Santos MJ; Physiology Department, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil., Souza Santos RA; Physiology Department, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil., Braga VA; Department of Biotechnology, Biotechnology Center, Federal University of Paraiba, João Pessoa, PB, Brazil., Santos SHS; Institute of Agricultural Sciences, Food Engineering College, Universidade Federal de Minas Gerais (UFMG), Montes Claros, Minas Gerais, Brazil; Lab. Health Science, PPGCS, Universidade Estadual de Montes Claros (Unimontes), Minas Gerais, Brazil. Electronic address: sergiosousas@hotmail.com.
Jazyk: angličtina
Zdroj: Life sciences [Life Sci] 2018 Sep 15; Vol. 209, pp. 341-348. Date of Electronic Publication: 2018 Aug 14.
DOI: 10.1016/j.lfs.2018.08.034
Abstrakt: Diabetic cardiomyopathy (DC) describes diabetes-associated changes in the structure and function of myocardium that are not directly linked to other factors such as hypertension. Currently there are some models of DC; however, they take a large time period to mimic key features. In the present study, we investigated the effects of a short-term high-fat/high salt diet (HFHS) treatment on myocardial function and structure, and vascular reactivity in C57BL/6 male mice. After 14 weeks HFHS induced hypertension (MAP = 144.95 ± 16.13 vs 92.90 ± 18.95 mm Hg), low glucose tolerance (AUC = 1049.01 ± 74.79 vs 710.50 ± 52.57 a.u.), decreased insulin sensitivity (AUC = 429.83 ± 35.22 vs 313.67 ± 19.55 a.u.) and increased adiposity (epididymal fat weight 0.96 ± 0.10 vs 0.59 ± 0.06 OW/BW × 10 2 ), aspects present in metabolic syndrome. Cardiac evaluation showed diastolic dysfunction (E/A ratio = 1.20 vs 1.90 u.a.) and cardiomyocyte hypertrophy (cardiomyocyte area = 502.82 ± 31.46 vs 385.58 ± 22.11 μm 2 ). Lastly, vascular reactivity was impaired with higher contractile response (136.10 ± 3.49 vs 120.37 ± 5.43%) and lower response to endothelium-dependent vasorelaxation (74.01 ± 4.35 vs 104.84 ± 3.57%). In addition, the diet was able to induce an inward coronary remodeling (vascular total area: SCNS 6185 ± 800.6 vs HFHS 4085 ± 213.7 μm 2 ). Therefore, we conclude that HFHS short-term treatment was able to induce metabolic syndrome-like state, cardiomyopathy and vascular injury working as an important tool to study cardiometabolic diseases.
(Copyright © 2018 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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