The ERM family member Merlin is required for endometrial gland morphogenesis.

Autor: Lopez EW; Department of Genetics, University of Texas, MD Anderson Cancer Center, Houston, TX 77030, USA; Graduate Program of Genetics and Epigenetics, The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, Houston, TX 77030, USA., Vue Z; Department of Genetics, University of Texas, MD Anderson Cancer Center, Houston, TX 77030, USA; Graduate Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA., Broaddus RR; Graduate Program of Genetics and Epigenetics, The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, Houston, TX 77030, USA; Department of Pathology, University of Texas, MD Anderson Cancer Center, Houston, TX 77030, USA., Behringer RR; Department of Genetics, University of Texas, MD Anderson Cancer Center, Houston, TX 77030, USA; Graduate Program of Genetics and Epigenetics, The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, Houston, TX 77030, USA; Graduate Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA., Gladden AB; Department of Genetics, University of Texas, MD Anderson Cancer Center, Houston, TX 77030, USA; Graduate Program of Genetics and Epigenetics, The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, Houston, TX 77030, USA. Electronic address: agladden@mdanderson.org.
Jazyk: angličtina
Zdroj: Developmental biology [Dev Biol] 2018 Oct 15; Vol. 442 (2), pp. 301-314. Date of Electronic Publication: 2018 Aug 15.
DOI: 10.1016/j.ydbio.2018.08.006
Abstrakt: Disruption of endometrial gland formation or function can cause female infertility. Formation of endometrial glands via tubulogenesis of luminal epithelial cells requires the establishment and maintenance of cell polarity and cell adhesion. The FERM domain-containing protein Merlin coordinates epithelial cell polarity and cell adhesion and is critical for epithelial tissue function in the skin and kidney. We now demonstrate a requirement for Merlin in endometrial gland development. Conditional deletion of Merlin in the endometrium results in female infertility caused by the absence of gland formation. Interestingly, we observed glandular epithelial markers within discrete groups of cells in the Merlin-deficient luminal epithelium. Wnt signaling, a pathway necessary for endometrial gland development is maintained in Merlin-deficient endometrium, suggesting the glandular fate program is active. Instead, we observe increased levels of apical actin and markers indicative of high membrane tension on the basal surface of the Merlin-deficient luminal epithelium. These findings suggest that the structural integrity of the luminal epithelium during gland formation is required for appropriate endometrial tubulogenesis and tissue function. Moreover, our work implicates Merlin-dependent regulation of mechanical tension in the proper formation of endometrial gland architecture and function.
(Copyright © 2018 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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