Desulfitobacterium contributes to the microbial transformation of 2,4,5-T by methanogenic enrichment cultures from a Vietnamese active landfill.

Autor: Lechner U; Institute of Biology/Microbiology, Martin-Luther University Halle-Wittenberg, Halle, Germany., Türkowsky D; Department of Molecular Systems Biology, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany., Dinh TTH; Vietnamese Academy of Science and Technology, Institute of Biotechnology, Hanoi, Vietnam., Al-Fathi H; Institute of Biology/Microbiology, Martin-Luther University Halle-Wittenberg, Halle, Germany., Schwoch S; Institute of Biology/Microbiology, Martin-Luther University Halle-Wittenberg, Halle, Germany., Franke S; Institute of Biology/Microbiology, Martin-Luther University Halle-Wittenberg, Halle, Germany., Gerlach MS; Institute of Biology/Microbiology, Martin-Luther University Halle-Wittenberg, Halle, Germany., Koch M; Institute of Chemistry/Food and Environmental Chemistry, Martin-Luther University Halle-Wittenberg, Halle, Germany., von Bergen M; Department of Molecular Systems Biology, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany., Jehmlich N; Department of Molecular Systems Biology, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany., Dang TCH; Vietnamese Academy of Science and Technology, Institute of Biotechnology, Hanoi, Vietnam.
Jazyk: angličtina
Zdroj: Microbial biotechnology [Microb Biotechnol] 2018 Nov; Vol. 11 (6), pp. 1137-1156. Date of Electronic Publication: 2018 Aug 16.
DOI: 10.1111/1751-7915.13301
Abstrakt: The herbicide 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) was a major component of Agent Orange, which was used as a defoliant in the Vietnam War. Little is known about its degradation under anoxic conditions. Established enrichment cultures using soil from an Agent Orange bioremediation plant in southern Vietnam with pyruvate as potential electron donor and carbon source were shown to degrade 2,4,5-T via ether cleavage to 2,4,5-trichlorophenol (2,4,5-TCP), which was further dechlorinated to 3,4-dichlorophenol. Pyruvate was initially fermented to hydrogen, acetate and propionate. Hydrogen was then used as the direct electron donor for ether cleavage of 2,4,5-T and subsequent dechlorination of 2,4,5-TCP. 16S rRNA gene amplicon sequencing indicated the presence of bacteria and archaea mainly belonging to the Firmicutes, Bacteroidetes, Spirochaetes, Chloroflexi and Euryarchaeota. Desulfitobacterium hafniense was identified as the dechlorinating bacterium. Metaproteomics of the enrichment culture indicated higher protein abundances of 60 protein groups in the presence of 2,4,5-T. A reductive dehalogenase related to RdhA3 of D. hafniense showed the highest fold change, supporting its function in reductive dehalogenation of 2,4,5-TCP. Despite an ether-cleaving enzyme not being detected, the inhibition of ether cleavage but not of dechlorination, by 2-bromoethane sulphonate, suggested that the two reactions are catalysed by different organisms.
(© 2018 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.)
Databáze: MEDLINE
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