The Development and Study of Recombinant Immunoglobulin A to Hemagglutinins of the Influenza Virus.

Autor: Aliev TK; Lomonosov Moscow State University, Department of Chemistry, Leninskie gory 1, bldg. 3, Moscow, 119991, Russia., Dement'yeva IG; Russian Research Center for Molecular Diagnostics and Therapy, Simferopolsky Blvd. 8, Moscow, 117149 , Russia., Toporova VA; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str. 16/10, Moscow, 117997, Russia., Argentova VV; Lomonosov Moscow State University, Faculty of Biology, Leninskie gory 1, bldg. 12, Moscow, 119991 , Russia., Pozdnyakova LP; Russian Research Center for Molecular Diagnostics and Therapy, Simferopolsky Blvd. 8, Moscow, 117149 , Russia., Bokov MN; Russian Research Center for Molecular Diagnostics and Therapy, Simferopolsky Blvd. 8, Moscow, 117149 , Russia., Votchitseva YA; Lomonosov Moscow State University, Faculty of Biology, Leninskie gory 1, bldg. 12, Moscow, 119991 , Russia., Dolgikh DA; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str. 16/10, Moscow, 117997, Russia.; Lomonosov Moscow State University, Faculty of Biology, Leninskie gory 1, bldg. 12, Moscow, 119991 , Russia., Varfolomeyev SD; Lomonosov Moscow State University, Department of Chemistry, Leninskie gory 1, bldg. 3, Moscow, 119991, Russia., Sveshnikov PG; Russian Research Center for Molecular Diagnostics and Therapy, Simferopolsky Blvd. 8, Moscow, 117149 , Russia., Kirpichnikov MP; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str. 16/10, Moscow, 117997, Russia.; Lomonosov Moscow State University, Faculty of Biology, Leninskie gory 1, bldg. 12, Moscow, 119991 , Russia.
Jazyk: angličtina
Zdroj: Acta naturae [Acta Naturae] 2018 Apr-Jun; Vol. 10 (2), pp. 30-36.
Abstrakt: We obtained recombinant variants of human antibody FI6 broadly specific to hemagglutinins of the influenza A virus. On the basis of a bi-promoter (CMV, hEF1-HTLV) vector, we developed genetic constructs for the expression of the heavy and light chains of the immunoglobulins of IgA1-, IgA2m1-, and IgG-isotypes. Following transfection and selection, stable Chinese hamster ovary (CHO) cell lines were produced. The antibodies of IgA1-, IgA2m1-, and IgG-isotypes were purified from culture media. We performed an immunochemical characterization and studied their interactions with influenza A strains of the H1N1- and H3N2-subtypes. It was shown that recombinant FI6 variants of the IgA-isotype retain the properties of the parental IgG antibody to demonstrate specificity to all the strains tested. The strongest binding was observed for the H1N1 subtype, which belongs to hemagglutinins of phylogenetic group I.
Databáze: MEDLINE